Chemosensitivity

A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity,Volume 2: In Vivo Models, Imaging, and Molecular Regulators contains cutting-edge protocols for classifying tumors into response categories and for customizing therapy to individuals. These readily reproducible techniques allow measurements of DNA damage, apoptotic cell death, and the molecular and cellular regulators of cytotoxicity, as well as in vivo animal modeling of chemosensitivity. A companion volume, Volume 1: In Vitro Assays contains in vitro and in vivo techniques to identify which new agents or combination of agents are effective for each type of tumor.

Includes supplementary material: sn.pub/extras

Texte du rabat
Chemosensitivity testing is an ex vivo means of determining or enhancing the cytotoxic and/or cytostatic, or apoptosis-inducing effects of anticancer drugs. In Chemosensitivity, leading researchers and physicians working in academia and biotech companies describe their best laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Volume 2: In Vivo Models, Imaging, and Molecular Regulators contains today's best protocols for classifying tumors into response categories and for customizing therapy to individuals. These techniques allow measurements of DNA damage, apoptotic cell death, and the molecular and cellular regulators of cytotoxicity, as well as in vivo animal modeling of chemosensitivity. Highlights include genomic and proteomic approaches to assess chemosensivity, in vivo imaging approaches to assess early response to therapy, and methods to statistically analyze data from in vivo therapy. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The authors also provide guidance on how best to analyze the data derived from the protocols. A companion volume, Volume 1: In Vitro Assays contains in vitro and in vivo techniques to identify which new agents or combination of agents are effective for each type of tumor.
Cutting-edge and highly practical, the two volumes of Chemosensitivity provide a comprehensive collection of readily reproducible techniques for the in vitro and in vivo screening of new agents and a set of proven approaches to understand mechanistically why certain cancer cell lines (in vitro) of tumors (in vivo) are more or less sensitive to a particular agent.

Contenu
Measurements of DNA Damage, Cell Death, and Regulators of Cytotoxicity.- In Vitro Micronucleus Technique to Predict Chemosensitivity.- Cell Cycle and Drug Sensitivity.- TUNEL Assay as a Measure of Chemotherapy-Induced Apoptosis.- Apoptosis Assessment by the DNA Diffusion Assay.- PARP Cleavage and Caspase Activity to Assess Chemosensitivity.- Diphenylamine Assay of DNA Fragmentation for Chemosensitivity Testing.- Immunodetecting Members of the Bcl-2 Family of Proteins.- Correlation of Telomerase Activity and Telomere Length to Chemosensitivity.- Application of Silicon Sensor Technologies to Tumor Tissue In Vitro.- Overview of Tumor Cell Chemoresistance Mechanisms.- Flow Cytometric Monitoring of Fluorescent Drug Retention and Efflux.- Flow Cytometric Measurement of Functional and Phenotypic P-Glycoprotein.- Measurement of Ceramide and Sphingolipid Metabolism in Tumors.- Genomics, Proteomics, and Chemosensitivity.- Gene Expression Profiling to Characterize Anticancer Drug Sensitivity.- Identifying Genes Related to Chemosensitivity Using Support Vector Machine.- Genetic Manipulation of Yeast to Identify Genes Involved in Regulation of Chemosensitivity.- Real-Time RT-PCR (Taqman®) of Tumor mRNA to Predict Sensitivity of Specimens to 5-Fluorouracil.- Use of Proteomics to Study Chemosensitivity.- In VIVO Animal Modeling of Chemosensitivity.- Clinically Relevant Metastatic Breast Cancer Models to Study Chemosensitivity.- Orthotopic Metastatic (MetaMouse®) Models for Discovery and Development of Novel Chemotherapy.- Preclinical Testing of Antileukemic Drugs Using an In Vivo Model of Systemic Disease.- Assessing Growth and Response to Therapy in Murine Tumor Models.- Evaluation of Chemosensitivity of Micrometastases with Green Fluorescent Protein Gene-Tagged Tumor Models inMice.- 99mTc-Annexin A5 Uptake and Imaging to Monitor Chemosensitivity.- Magnetic Resonance Imaging of Tumor Response to Chemotherapy.- Metabolic Monitoring of Chemosensitivity with 18FDG PET.