Brain Development

This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizingrecent technical advances in molecular genetics for the study of the brain.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

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Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra.- Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis .- Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis .- Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System.- Immunostaining of the Embryonic and Larval Drosophila Brain.- Non-Fluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila.- Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling.- Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update.- Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain.- CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila.- The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development.- A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum.- Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium .- X-Ray Microscopy of the Larval Crustacean Brain.- Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain.- Immunostainings in Nervous System Development of the Nematode C. elegans .- Methods in Brain Development of Molluscs.- A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization.- Spawning Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum .- In Situ Hybridization and Immunostaining of Xenopus Brain.-Morpholino Studies in Xenopus Brain Development.- Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish.- Live Morphometric Classification of Sensory Neurons in Larval Zebrafish.- Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain.- Gene Silencing in Chicken Brain Development.- Transplantation of Neural Tissue: Quail-Chick Chimeras.- Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development.- The Cre/Lox System to Assess the Development of the Mouse Brain.- In Utero Electroporation to Study Mouse Brain Development.