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Unnatural Amino Acid Incorporation and Click Chemistry

  • Couverture cartonnée
  • 52 Nombre de pages
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Seminar paper from the year 2014 in the subject Chemistry - Bio-chemistry, grade: 1,0, LMU Munich (Chemie), language: English, abs... Lire la suite
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Description

Seminar paper from the year 2014 in the subject Chemistry - Bio-chemistry, grade: 1,0, LMU Munich (Chemie), language: English, abstract: In the present work a modified version of yellow fluorescent protein containing an unnatural structural homologue of the natural amino acid pyrrolysine with a norbornene moiety was produced by expression in Escherichia coli. The incorporation of the unnatural amino acid was achieved by amber stop codon suppression method. A bio-othogonal click reaction was performed, binding a synthetic fluorescent dye to the modified protein. All steps towards necessary for obtaining the genetically modified organism were performed and documented. The artificial amino acid, as well as the dye used in the click reaction were synthetically prepared. The success of the project was demonstrated by LC/MS studies of the products. Fluorescence spectroscopy of click reaction product and the protein was performed, but no conclusive proof of FRET effects could as yet be made. This point remains of interest for future studies.

Informations sur le produit

Titre: Unnatural Amino Acid Incorporation and Click Chemistry
Auteur:
Code EAN: 9783668663473
ISBN: 978-3-668-66347-3
Format: Couverture cartonnée
Genre: Chimie
nombre de pages: 52
Poids: 88g
Taille: H3mm x B210mm x T148mm
Année: 2018

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