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Chemical RNA Synthesis

  • Agrafé
  • 16 Nombre de pages
Internship Report from the year 2014 in the subject Chemistry - Bio-chemistry, grade: 1,0, Free University of Berlin (Institut für... Lire la suite
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Description

Internship Report from the year 2014 in the subject Chemistry - Bio-chemistry, grade: 1,0, Free University of Berlin (Institut für Chemie und Biochemie), course: Methodenmodul Nukleinsäuren, language: English, abstract: Oligonucleotides that are used in various experiments such as PCR, Next Generation Sequencing or quantitative PCR can be synthesized chemically. In contrast to the natural 5' 3' synthesis, the chemical synthesis generates the strand in a 3' 5' direction. The first base is coupled covalently to Controlled Pore Glass (CPG). The cyclic elongation reaction consists of five steps: detritylation, tetrazolactivation, coupling, capping and oxidation (figure 1). The synthesis is automated by a synthesizing machine. Here, we exemplarily synthesized an RNA 20mer with and without a 5'-dimethoxytrityl protection group, respectively, in a 0.2 mimol synthesis scale.

Informations sur le produit

Titre: Chemical RNA Synthesis
Auteur:
Code EAN: 9783656823438
ISBN: 365682343X
Format: Agrafé
Genre: Chimie
nombre de pages: 16
Poids: 45g
Taille: H211mm x B148mm x T3mm
Année: 2014
Auflage: 1. Auflage.

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