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Wnt Signaling

  • Fester Einband
  • 198 Seiten
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Beschreibung

Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Wnt Signaling: Methods and Protocols aims to support researchers in understanding Wnt signal transduction in any effort to improve outcomes in regenerative medicine and cancer management.

Includes cutting-edge methods and protocols for the study of Wnt signaling

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts



Inhalt
1. Visualizing Wnt Palmiteoylation in Single CellsXinxin Gao and Rami N. Hannoush2. Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition ReactionRubina Tuladhar, Nageswari Yarravarapu, and Lawrence Lum3. Biochemical Methods to Analyze Wnt Protein SecretionKathrin Glaeser, Michael Boutros, and Julia Christina Gross4. Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and NotumXinjun Zhang and Xi He5. Probing Wnt Receptor Turnover: A Critical Regulatory Point of Wnt PathwayXiaomo Jiang and Feng Cong6. A Simple Method to Assess Abundance of the b-Catenin Signaling Pool in CellsAnnette S. Flozak, Anna P. Lam, and Cara J. Gottardi7. Wnt-Dependent Control of Cell Polarity in Cultured CellsKristin B. Runkle and Eric S. Witze8. The Use of Chick Embryos to Study Wnt Signaling/GradientsLisa M. Galli, Tiffany Barnes, and Laura W. Burrus9. Monitoring Wnt Signaling in Zebrafish Using Fluorescent BiosensorsNicola Facchinello, Marco Schiavone, Andrea Vettori, Francesco Argenton, and Natascia Tiso10. Biochemical Analysis of Tankyrase Activity in Zebrafish In Vitro and In VivoJesung Moon and James F. Amatruda11. Reconstitution of the Cytoplasmic Regulation of the Wnt Signaling Pathway Using Xenopus Egg ExtractsAnnastasia Simone Hyde, Brian I. Hang, and Ethan Lee12. Delivery of the Porcupine Inhibitor WNT974 in MiceLi-shu Zhang and Lawrence Lum13. Use of Primary Calvarial Osteoblasts to Evaluate the Function of Wnt Signaling in OsteogenesisZhendong A. Zhong, Nicole J. Ethen, and Bart O. Williams14. Monitoring Wnt/b-Catenin Signaling in SkinAmy Ku, Qi Miao, and Hoang Nguyen<15. The Generation of Organoids for Studying Wnt SignalingJarno Drost, Benedetta Artegiani, and Hans Clevers16. Methods to Manipulate and Monitor Wnt Signaling in Human Pluripotent Stem CellsIan Huggins, David Brafman, and Karl Willert17. Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined ConditionsXiaoping Bao, Xiaojun Lian, and Sean P. Palecek

Produktinformationen

Titel: Wnt Signaling
Untertitel: Methods and Protocols
Editor:
EAN: 9781493963911
ISBN: 978-1-4939-6391-1
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 198
Größe: H18mm x B262mm x T182mm
Jahr: 2016
Untertitel: Englisch
Auflage: 1st ed. 2016

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