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Vaccine Design

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This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Chapters detail protocols for... Weiterlesen
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This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Chapters detail protocols for developing novel vaccines against infectious bacteria, viruses, fungi, and parasites for humans and animals. Volume 1: Vaccines for Human Diseases has an introductory section on how vaccines impacted diseases, the immunological mechanism of vaccines, future challenges for vaccinologists, and current trends in vaccinology. The design of human vaccines for viral, bacterial, fungal, parasitic and prion diseases as well as vaccines for drug abuse, allergy, and tumor vaccines are also described in this volume. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Vaccine Design: Methods and Protocols, Volume 1: Vaccines

for Human Diseases aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Clinical Impact of Vaccine Development.- Future Challenges for Vaccinologists.- Principles of Vaccination.- Reverse Vaccinology: The Pathway from Genomes and Epitope Predictions to Tailored Recombinant Vaccines.- Systems Vaccinology: Applications, Trends, and Perspectives.- Proteomic Monitoring of B Cell Immunity.- Development of Rabies Virus-Like Particles for Vaccine Applications: Production, Characterization and Protection Studies.- Analytic Vaccinology: Antibody-Driven Design of a Human Cytomegalovirus Subunit Vaccine.- Generation of a Single-Cycle Replicable Rift Valley Fever Vaccine.- Application of Droplet Digital PCR to Validate Rift Valley Fever Vaccines.- Methods to Evaluate Novel Hepatitis C Virus Vaccines.- Designing Efficacious Vesicular Stomatitis Virus-Vectored Vaccines against Ebola Virus.- Assessment of Functional Norovirus Antibody Responses by Blocking Assay in Mice.- Development of a SARS Coronavirus Vaccine from Recombinant Spike Protein plus Delta Inulin Adjuvant.- Generation and Characterization of a Chimeric Tick-Borne Encephalitis Virus Attenuated Strain ChinTBEV.- Single-Vector, Single-injection Recombinant Vesicular Stomatitis Virus Vaccines against High Containment Viruses.- Reverse Genetics Approaches to Control Arenavirus.- DNA Vaccines: A Strategy for Developing Novel Multivalent TB Vaccines.- Overcoming Enterotoxigenic Escherichia coli Pathogen Diversity: Translational Molecular Approaches to Inform Vaccine Design.- Design and Purification of Subunit Vaccines for Prevention of Clostridium difficile Infection.- The Design of a Clostridium difficile Carbohydrate-based Vaccine.- Murine Models of Bacteremia and Surgical Wound Infection for the Evaluation of Staphylococcus aureus Vaccine Candidates.- Using MHC Molecules to define a Chlamydia T Cell Vaccine .- An Approach to Identify and Characterize a Subunit Candidate Shigella Vaccine Antigen.- Approach to the Discovery, Development and Evaluation of a Novel Neisseria meningitidis Serogroup B Vaccine.- Anti-Lyme Subunit Vaccines: Design and Development of Peptide-based Vaccine Candidates.- Assessment of Live Plague Vaccine Candidates.- Highly Effective Soluble and Bacteriophage T4 Nanoparticle Plague Vaccines against Yersinia pestis.- Development of Structure-based Vaccines for Ehrlichiosis.- Dendritic Cell-based Vaccine against Fungal Infection.- Flow Cytometric Analysis of Protective T-cell Response against Pulmonary Coccidioides Infection.- High-Density Peptide Arrays for Malaria Vaccine Development.- Development and Assessment of Transgenic Rodent Parasites for the Pre-clinical Evaluation of Malaria Vaccines.- DNA Integration in Leishmania Genome: an Application for Vaccine Development and Drug Screening.- Methods to Evaluate the Preclinical Safety and Immunogenicity of Genetically Modified Live Attenuated Leishmania Parasite Vaccines.- The Use of Microwave Assisted Solid Phase Peptide Synthesis and Click Chemistry for the Synthesis of Vaccine Candidates against Hookworm Infection.- Methods and Protocols for Developing Prion Vaccines.- Ricin-Holotoxin Based Vaccines: Induction of Potent Ricin Neutralizing Antibodies.- Synthesis of Hapten-protein Conjugate Vaccines with Reproducible Hapten Densities.- Production of Rice Seed-based Allergy Vaccines.- Allergy Vaccines Using a Mycobacterium-Secreted Antigen, Ag85B, and an IL-4 Antagonist.- Development of House Dust Mite Vaccine.- Cancer Vaccines: A Brief Overview.- Dendritic Cell Vaccines.- Peptide-based Cancer Vaccine Strategies and Clinical Results.- Preconditioning Vaccine Sites for mRNA--Transfected Dendritic Cell Therapy and Antitumor Efficacy.- Development of Antibody based Vaccines Targeting the Tumor Vasculature.


Titel: Vaccine Design
Untertitel: Methods and Protocols: Volume 1: Vaccines for Human Diseases
EAN: 9781493933853
ISBN: 149393385X
Format: Fester Einband
Herausgeber: Springer New York
Genre: Medizin
Anzahl Seiten: 896
Gewicht: 1847g
Größe: H260mm x B183mm x T53mm
Auflage: 1st ed. 2016

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