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Tumor Angiogenesis Assays

  • Kartonierter Einband
  • 208 Seiten
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This volume aims to provide a range of methods and protocols for studying tumor angiogenesis in vitro and in vivo to reflect advan... Weiterlesen
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This volume aims to provide a range of methods and protocols for studying tumor angiogenesis in vitro and in vivo to reflect advances in the field. The chapters in this book cover topics such as: morphological aspects of tumor angiogenesis, aortic ring assay and its use for the study of tumor angiogenesis, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and uncovering metabolic effects of anti-angiogenic therapy in tumors by induced metabolic bioluminescence imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Tumor Angiogenesis Assays: Methods and Protocols is a valuable resource for anyone interested in tumor angiogenesis assay research.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts


1. The Discovery of Tumor Angiogenesis Factors: An Historical Overview
Domenico Ribatti

2. Morphological Aspects of Tumor Angiogenesis
Rusland Hlushchuk, Sébastien Barré , Valentin Djonov

3. Exosomes in Tumor Angiogen esis
Karma Z. Salem, Michele Moschetta, Antonio Sacco, Luisa Imberti, Giuseppe Rossi, Irene M. Ghobrial, Salomon Manier, and Aldo M. Roccaro

4. Co-Culture Assays for Endothelial Cells: Mural Cells Interactions
Diana M. Sánchez-Palencia, Alex Bigger-Allen, Magali Saint-Geniez, Joseph F. Arboleda-Velásquez, Patricia A. D'Amore

5. Characterization of Cancer Stem Cells
Stefania Orecchioni and Francesco Bertolini

6. The Aortic Ring Assay and its Use for the Study of Tumor Angiogenesis
Alfred C. Aplin and Roberto F. Nicosia

7. Surface Plasmon Resonance Analysis of Heparin-Binding Angiogenic Growth Factors
Antonella Bugatti and Marco Rusnati

8. An Ex Vivo Tissue Culture Model for Anti-Angiogenic Drug Testing
Mohammad S. Azimi, Michelle Lacey, Debasis Mondal, and Walter L. Murfee

9. The Chorioallantoic Membrane of the Chick Embryo to Assess Tumor Formation and Metastasis
Anne Herrmann, Diana Moss, and Violaine Sée

10. Transgenic Zebrafish
Xiaowen Chen, Dafne Gays, and Massimo M. Santoro

11. High Resolution Intravital Microscopy of Tumor Angiogenesis
Ann L.B. Seynhaeve and Timo L.M. ten Hagen

12. Subcutaneous Murine Xenograft Models: A Critical Tool for Studying Human Tumor Growth and Angiogenesis In Vivo
Katharina M. Schmidt, Edward K. Geissler, and Sven A. Lang

13. Orthotopic Models of Ovarian Cancer
Alessandra Decio and Raffaella Giavazzi

14. The Rip1Tag2 Transgenic Mouse Model
Ruben Bill and Gerhard Christophori

15. Tumor Blood Vessel Visualization
Clarissa Gillmann and Tobias Bäeuerle

16. Uncovering Metabolic Effects of Anti-Angiogenic Therapy in Tumors by Induced Metabolic Bioluminescence Imaging
Stefano Indraccolo, Stefan Walenta, and Wolfgang Mueller-Klieser

17. Quantitation of Tumor Angiogenesis In Vitro: An All-In-One Angiogenesis Assay
Mahtab Bahramsoltani and Ward De Spiegelaere


Titel: Tumor Angiogenesis Assays
Untertitel: Methods and Protocols
EAN: 9781493981496
ISBN: 1493981498
Format: Kartonierter Einband
Herausgeber: Springer New York
Anzahl Seiten: 208
Gewicht: 402g
Größe: H254mm x B178mm x T11mm
Jahr: 2018
Untertitel: Englisch
Auflage: Softcover reprint of the original 1st ed. 2016

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