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Transgenesis Techniques

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Leseprobe
This fully updated and expanded edition details the transgenic techniques currently used to modify the genome. It explores classic... Weiterlesen
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Beschreibung

This fully updated and expanded edition details the transgenic techniques currently used to modify the genome. It explores classic procedures as well as cutting-edge practices involving microinjection, site-specific recombination systems, and cryopreservation.

One of the major challenges currently facing the scientific community is to understand the function of the multitude of protein-coding genes that were revealed when the human genome was fully sequenced. In Transgenesis Techniques: Principles and Protocols, Third Edition, experts in the field fully update and expand upon the previous edition in order to detail the transgenic techniques currently used to modify the genome. The volume explores classic procedures to genetically modify mice and other model organisms, as well as cutting-edge practices involving microinjection, site-specific recombination systems, cryopreservation, and many other topics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and state-of-the-art, Transgenesis Techniques: Principles and Protocols, Third Edition is a comprehensive practical guide to the generation of transgenic animals and an invaluable source of information in any lab currently involved in transgenic techniques.



Provides state-of-the-art alongside the classics, i.e. classic techniques have been brought up to date, and are described along with modern state-of-the-art transgenesis technologies

Covers a wide range of organisms, including Drosophila, C. elegans, Xenopus, zebrafish, rat and mouse

Presents an accessible, user-friendly guide designed to inform and guide researchers currently involved in transgenic techniques and those new to the field

Includes essential background information for scientists who work with genetically modified organisms but are not routinely involved in their generation

Provides an essential blend of theory and practice

Contributions by experienced authors from world renowned institutes

Acts as an essential technical guide complete with lots of trouble shooting from researchers who have 'been there and done it'



Inhalt
Part I: Transgenesis in Various Model Systems 1. Transgenesis in Drosophila melanogaster Leonie Ringrose 2. Transgenesis in Caenorhabditis elegans Matthias Rieckher, Nikos Kourtis, Angela Pasparaki, and Nektarios Tavernarakis 3. Transgenesis in Zebrafish with the Tol2 Transposon System Maximiliano L. Suster, Hiroshi Kikuta, Akihiro Urasaki, Kazuhide Asakawa, and Koichi Kawakami 4. Generation of Transgenic Frogs Jana Loeber, Fong Cheng Pan, and Tomas Pieler 5. Pronuclear DNA Injection for the Production of Transgenic Rats Jean Cozzi, Ignacio Anegon, Valérie Braun, Anne-Catherine Gross, Christel Merrouche, and Yacine Cherifi Part II: Transgenesis in the Mouse 6. Cell-Type Specific Transgenesis in the Mouse James Gulick and Jeffrey Robbins 7. Transgene Design and Delivery into the Mouse Genome: Keys to Success Lydia Teboul 8. Overexpression Transgenesis in Mouse: Pronuclear Injection Wendy J.K. Gardiner and Lydia Teboul 9. Gene Targeting Vectors J. Simon C. Arthur and Victoria A. McGuire 10. Gene Trap: Knockout on the Fast Lane Melanie Ullrich and Kai Schuh 11. Culture of Murine Embryonic Stem Cells Ivana Barbaric and T. Neil Dear 12. Targeting Embryonic Stem Cells Roland H. Friedel 13. Generation of Chimeras by Microinjection Anne Plück and Christian Klasen 14. Generation of Chimeras by Morula Aggregation Anne Plück and Christian Klasen 15. Surgical Techniques for the Generation of Mutant Mice Anne Plück and Christian Klasen 16. Site-specific Recombinases for Manipulation of the Mouse Genome Marie-ChristineBirling, Françoise Gofflot, and Xavier Warot 17. Cre Transgenic Mouse Lines Xin Wang 18. Large-Scale Mouse Mutagenesis Elizabeth J. Cartwright 19. Dedicated Mouse Production and Husbandry Lucie Vizor and Sara Wells 20. Biological Methods for Archiving and Maintaining Mutant Laboratory Mice: Part I - Conserving Mutant Strains Martin D. Fray 21. Biological Methods for Archiving and Maintaining Mutant Laboratory Mice: Part II - Recovery and Distribution of Conserved Mutant Strains Martin D. Fray

Produktinformationen

Titel: Transgenesis Techniques
Untertitel: Principles and Protocols
Editor:
EAN: 9781603270182
ISBN: 1603270183
Format: Fester Einband
Herausgeber: Springer-Verlag GmbH
Genre: Medizin
Veröffentlichung: 01.07.2009
Anzahl Seiten: 336
Gewicht: 1033g
Größe: H266mm x B198mm x T26mm
Jahr: 2009
Untertitel: Englisch
Auflage: 3rd ed

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