Toll-Like Receptors

This second edition volume expands on the first edition with new developments on Toll-Like Receptors (TLRs) controlling events such as cross-priming of associated pattern recognition receptors, post-transcriptional regulation, interaction with other cellular and biologic systems, and cancer progression. This book is divided into five sections: Part I outlines methods for TLR detection, interaction, and intracellular trafficking; Part II describes methods and assays to investigate how TLRs cross-prime other pattern recognition receptors, including intracellular DNA receptors and inflammasome formation; Part III highlights RNA regulation, detailing how TLRs can induce RNA transcripts and molecules such as lncRNAs and microRNAs; Part IV explores TLR detection and activation in systems such as epithelial barrier function, metabolism and the circadian clock, as well as cellular systems including T and B lymphocytes; and Part V describes models to delineate the role of TLRs in diseases such as dermatitis, arthritis, and gastric cancer. Written in the highly successful Methods in Molecular Biology series format, each chapter contains a summary, a list of required materials, step-by-step, readily reproducible laboratory protocols, useful notes to investigate TLRs in cell culture, systems and disease, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Toll-Like Receptors: Methods and Protocols, Second Edition is a valuable resource to any immunologist, molecular or medical biologist working in a laboratory setting. It will add skill to both students and the more advanced molecular biologist who wishes to learn a new technique or move to a different area within their current repertoire of practical knowledge.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt
Toll-Like Receptors: Ligands, Cell-Based Models, and Readouts for Receptor Action.- Bioinformatic Analysis of Toll-Like Receptor Sequences and Structures.- Toll-Like Receptor Interactions Measured by Microscopic and Flow Cytometric FRET.- Using Confocal Microscopy to Investigate Intracellular Trafficking of Toll-Like Receptors.- Assessing the Inhibitory Activity of Oligonucleotides on TLR7 Sensing.- Methods for Delivering DNA to Intracellular Receptors.- Detection of Interaction between Toll-Like Receptors and other Transmembrane Proteins by Co-Immunoprecipitation Assay.- Flow Cytometry-Based Bead-Binding Assay for Measuring Receptor Ligand Specificity.- Measuring Monomer-to-Filament Transition of MAVS as an In Vitro Activity Assay for RIG-I-Like Receptors.- Co-Transcriptomic Analysis by RNA Sequencing to Simultaneously Measure Regulated Gene Expression in Host and Bacterial Pathogen.- Simple Methods to Investigate MicroRNA Induction in Response to Toll-Like Receptors.- Determining the Function of Long Noncoding RNA in Innate Immunity.- Analysis of Post-Transcriptional Gene Regulation of Nod-Like Receptors via the 3'UTR.- TLR Function in Murine CD4 + T Lymphocytes and their Role in Inflammation.- Flow Cytometry Analysis of B Cell Activation and Antibody Responses Induced by Toll-Like Receptors.- Toll-Like Receptor-Dependent Immune Complex Activation of B Cells and Dendritic Cells.- Analysis of TLR-Induced Metabolic Changes in Dendritic Cells Using the Seahorse XF e 96 Extracellular Flux Analyzer.- Toll-Like Receptor Signaling and the Control of Intestinal Barrier Function.- Understanding the Role of Cellular Molecular Clocks in Controlling the Innate Immune Response.- Methods to Investigate the Role of Toll-Like Receptors in Allergic Contact Dermatitis.- Allergens and Activation of the Toll-Like Receptor Response.- Investigating the Role of Toll-Like Receptorsin Models of Arthritis.- Delineating the Role of Toll-Like Receptors in the Neuro-Inflammation Model EAE.- The Use of MiRNA Antagonists in the Alleviation of Inflammatory Disorders.- Investigating the Role of Toll-Like Receptors in Mouse Models of Gastric Cancer. <p