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Tissue Microarrays

  • Kartonierter Einband
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Today, tissue microarray methodology is a modern, high-end technique which allows us to link clinical data to tissues combined on ... Weiterlesen
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Beschreibung

Today, tissue microarray methodology is a modern, high-end technique which allows us to link clinical data to tissues combined on one slide. Here, top researchers offer insights into the complexities of the most popular assays used for in-situ tissue analysis.


The tissue microarray (TMA) method presents as a modern high technology, although its roots go back to the 80s when researchers frst started to combine several small pieces of tissues into so-called sausage blocks. In this respect, the TMA invention was not frstly characterized by technical improvements, but its true novelty was to link clinical data to the tissues that were combined on one slide. The very high number of tissues that can be included into one TMA, the small size and regular shape of the tissue spots, the preser- tion of integrity of the donor tissue blocks, and the highly organized array pattern that allows for reliable allocation of clinical data to individual tissue spots made it a discrete technique with unique features. When the TMA technology was developed 12 years ago, its beneft was controversially debated. While many researchers welcomed the method enthusiastically, there were c- cerns by others that results obtained from the small tissue cores used for TMA making would not be suffciently representative of the donor tissues. Meanwhile, the increasing use of this technology has imposingly demonstrated its tremendous utility in research. In fact, basically all clinically relevant associations between molecular markers and clinical endpoints could be reproduced using only one single 0. 6 mm core per tissue sample so that TMAs have nowadays become a standard tool allowing for a new dimension of tissue analysis.

From the reviews:

This book is the first to attempt to summarise the field of Tissue Microarrays (TMAs) since the first report of the technology in 1998. relevant to the clinician-scientist who may be considering high-throughput analyses of genes and proteins from a large cohort. In summary, this book is crammed with incredibly detailed technical advice and even advice on whether to drill or cut the recipient paraffin blocks. very readable, very informative and an excellent introduction to the gentle art of TMAs. (Cris dos Remedios and Sean Lal, Proteomics, Vol. 11, 2011)

Klappentext
Today's tissue microarray (TMA) method presents as a modern high-tech technology, one which allows for the linking of clinical data to the tissues that are combined on one slide. In Tissue Microarrays: Methods and Protocols, expert researchers explore the current world of TMA making and TMA applications, providing insight into the inherent and complex aspects of the most popular assays used for in-situ tissue analysis. Chapters examine the range of TMA techniques that allow for a large number of tissues to be included in one TMA, preserve the integrity of donor tissue blocks, and present a highly organized array pattern that allows for the reliable allocation of clinical data to individual tissue spots. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Contemporary and ground-breaking, Tissue Microarrays: Methods and Protocols serves as an essential handbook for pathologists, molecular biologists, researchers in the life sciences, as well as physicians, a reflection of the various applications of current TMA technology.

Inhalt

Tissue Microarrays: Methods and Protocols
Editor Ronald Simon

Table of Contents:


Table of Contents

Preface

Contributors

1. Applications of tissue microarray technology
Ronald Simon

2. Quality aspects of TMA analysis
Pierre Tennstedt and Guido Sauter

3. Representativity of TMA studies
Guido Sauter

4. Recipient block TMA technique
Martina Mirlacher and Ronald Simon

5. Protocol for constructing tissue arrays by cutting edge matrix assembly
Thai Hong Tran, Justin Lin, Ashley Brooke Sjolund, Fransiscus Eri Utama, and Hallgeir Rui

6. Hypodermic needle without recipient paraffin block technique
Andréa Rodrigues Cordovil Pires and Simone Rabello de Souza

7. Resin Technologies: Constructing and staining of resin TMAs
William J Howat and Susan J Wilson

8. Tissue microarrays from frozen tissues - OCT technique
Marlena Schoenberg Fejzo and Dennis J. Slamon

9. An Alternative Technology to Prepare Tissue Microarray Using Frozen Tissue Samples
Zhongting Hu, Elbert Chang, Melissa Hodeib, and Joseph D. Abad

10. Building "Tissue" Microarrays from Suspension Cells
Shuchun Zhao and Yasodha Natkunam

11. TMAs from Biopsy specimens
Milton W. Datta and André A. Kajdacsy-Balla,

12. Immunohistochemical analysis of tissue microarrays
Ronald Simon, Martina Mirlacher and Guido Sauter

13. DNA copy number analysis on Tissue Microarrays
Anne Kallioniemi

14. RNA expression analysis on formalin-fixed paraffin-embedded (FFPE) tissue in TMA format by RNA in situ hybridization
Jürgen Veeck and Edgar Dahl

15. Automated analysis of Tissue Microarrays
Marisa Dolled-Filhart, Mark Gustavson, Robert L. Camp, David L. Rimm, John L. Tonkinson, and Jason Christiansen

16. Digital microscopy for boosting database integration and analysis in TMA studies
Tibor Krenacs, L. Ficsor, V. S. Varga , V. Angeli , and B. Molnar

17. From Gene to Clinic: TMA based clinical validation of molecular markers in prostate cancer

T. Schlomm, F. K. Chun, and A.Erbersdobler

Produktinformationen

Titel: Tissue Microarrays
Untertitel: Methods and Protocols
Editor:
EAN: 9781493961030
ISBN: 1493961039
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 204
Gewicht: 395g
Größe: H254mm x B178mm x T11mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2010

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