The Renin-Angiotensin-Aldosterone System

This volume discusses protocols that aid in measuring different components in the renin-angiotensin-aldosterone system (RAAS). The book also looks at the methods used to assess angiotensin peptides and discerning the influence of RAAS components on different mammalian diseases. The chapters cover topics such as the use of fluorescent substrate to measure ACE2 activity in the mouse abdominal aorta, blood pressure monitoring using the radio telemetry method, and the analysis of angiotensin metabolism in the kidney using mass spectrometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and comprehensive, The Renin-Angiotensin-Aldosterone Systems: Methods and Protocols is a valuable resource that providesscientists and researchers with the best approaches to examine RAAS.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

A Brief Introduction into the Renin-Angiotensin-Aldosterone System: New and Old Techniques.- A Color Segmentation Based Method to Quantify Atherosclerotic Lesion Compositions with Immunostaining.- Assessment of Protein Carbonylation and Protein Tyrosine Phosphatase (PTP) Oxidation in Vascular Smooth Muscle Cells (VSMCs) using Immunoblotting Approaches.- Methods for Studying the Role of RAAS in the Modulation of Vascular Repair-Relevant Functions of Stem/Progenitor Cells.- Use of a Fluorescent Substrate to Measure ACE2 Activity in the Mouse Abdominal Aorta.- Measuring Blood Pressure using a Non-Invasive Tail Cuff Method in Mice.- Blood Pressure Monitoring using Radio Telemetry Method in Mice.- Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry.- Assessment of the Renin-Angiotensin System in Cellular Organelle: New Arenas for Study in the Mitochondria.- Comprehensive Assessments of Energy Balance in Mice.- In Vitro Assays to Determine Smooth Muscle Cell Hypertrophy, Protein Content, and Fibrosis.- A New Mouse Model for Introduction of Aortic Aneurysm by Implantation of Deoxycorticosterone Acetate Pellets or Aldosterone Infusion in the Presence of High Salt.- Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors.- A Primer to Angiotensin Peptide Isolation, Stability, and Analysis by Nano-Liquid Chromatography with Mass Detection.- Analysis of Angiotensin Metabolism in the Kidney using Mass Spectrometry.