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The Plant Cell Wall

  • Kartonierter Einband
  • 324 Seiten
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This book details current methods for investigating aspects of the plant cell wall including its structure, biochemical compositio... Weiterlesen
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This book details current methods for investigating aspects of the plant cell wall including its structure, biochemical composition, and metabolism, drawing on a range of techniques involving plant tissue culture which can be applied to many related studies.

The cell wall and its constituent polysaccharides and proteins control nearly all plant-based biological and biophysical processes. Understanding the cell wall is, therefore, not only fundamental to the plant sciences but is also pertinent to aspects of human and animal nutrition and health as well as plant-microbe and plant-animal interactions. In The Plant Cell Wall: Methods and Protocols, experts in the field describe detailed methods which are currently being applied to investigate the many aspects of the plant cell wall including its structure, biochemical composition, and metabolism. The book delves into a range of techniques involving plant tissue culture, which can be applied to investigating cell wall structure and metabolism, methods directed towards structural analysis and occurrence of carbohydrates, the development and use of microscopy-based tools and techniques, procedures which measure the physical properties of the wall, and methods based on the application of molecular genetic approaches. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, The Plant Cell Wall: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.

Features many recently developed methods, becoming more widely used with the development of advanced instrumentation and technology

Contains detailed protocols to study plant cell wall structure, biochemical composition, and metabolism, to name a few areas

Includes expert tips and vital implementation advice


1. Plant Tissue Cultures

Anna Kärkönen, Arja Santanen, Kuninori Iwamoto, and Hiroo Fukuda

2. Computerized Molecular Modelling of Carbohydrates

Alfred D. French and Glenn P. Johnson

3. Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS

Markus Günl, Florian Kraemer, and Markus Pauly

4. High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors

Stephen C. Fry

5. Carbohydrate Gel Electrophoresis

Florence Goubet, Paul Dupree, and Katja Salomon Johansen

6. Capillary Electrophoresis with Detection by Laser-Induced Fluorescence

Andrew Mort and Xiangmei Wu

7. Monoclonal antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls

Cécile Hervé, Susan E. Marcus, and J. Paul Knox

8. Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays

Iben Sørensen and William G.T. Willats

9. Electron Tomography and Immunogold Labelling as Tools to Analyze de novo Assembly of Plant Cell Walls

Marisa S. Otegui

10. Analyzing Cellulose Biosynthesis with Confocal Microscopy

Meera Nair and Seth DeBolt

11. Visual Mapping of Cell Wall Biosynthesis

Yumiko Sakuragi, Morten H.H. Nørholm, and Henrik V. Scheller

12. Atomic Force Microscopy of Plant Cell Walls

Andrew R. Kirby

13. Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls

Tracey J. Bootten, Philip J. Harris, Laurence D. Melton, and Roger H. Newman

14. Formation of Cellulose-Based Composites w ith Hemicelluloses and Pectins Using Gluconacetobacter Fermentation

Deirdre Mikkelsen and Michael J. Gidley

15. Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis

Derek T.A. Lamport, Li Tan, and Marcia J. Kieliszewski

16. New Insights into the Control of Cell Growth

Claudia Blaukopf, Matthäus Z. Krol, and Georg J. Seifert

17. Extraction and Detection of Arabinogalactan Proteins

Zoë A. Popper

18. Characterization of the Plant Cell Wall Proteome Using High Throughput Screens

Sang-Jik Lee and Jocelyn K.C. Rose

19. Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens

Alison W. Roberts, Christos S. Dimos, Michael J. Budziszek, Jr., Chessa A. Goss, and Virginia Lai

20. Measuring In vitro Extensibility of Growing Plant Cell Walls

Daniel J. Cosgrove


Titel: The Plant Cell Wall
Untertitel: Methods and Protocols
EAN: 9781493961795
ISBN: 1493961799
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 324
Gewicht: 612g
Größe: H254mm x B178mm x T17mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2011

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