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The Nuclear Envelope

  • Fester Einband
  • 540 Seiten
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This volume provides a wide range of protocols used in studying the nuclear envelope, with special attention to the experimental a... Weiterlesen
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This volume provides a wide range of protocols used in studying the nuclear envelope, with special attention to the experimental adjustments that may be required to successfully investigate this complex organelle in cells from various organisms. The Nuclear Envelope: Methods and Protocols is divided into five sections: Part I Nuclear Envelope Isolation; Part II Nuclear Envelope Protein Interactions, Localization, and Dynamics; Part III Nuclear Envelope Interactions with the Cytoskeleton; Part IV Nuclear Envelope-Chromatin Interactions; and Part V Nucleo-Cytoplasmic Transport. Many of the modifications discussed in this book have only been circulated within laboratories that have conducted research in this field for many years. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, The Nuclear Envelope: Methods and Protocols is a timely resource for researchers who have joined this dynamic and rapidly growing field.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Part I Nuclear Envelope Isolation Isolation, Proteomic Analysis and Microscopy Confirmation of the Liver Nuclear Envelope Proteome Nadia Korfali, Laurence Florens, and Eric C. Schirmer Exploring the Protein Composition of the Plant Nuclear Envelope Xiao Zhou, Kentaro Tamura, Katja Graumann, and Iris Meier High Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes Samson Obado, Mark C. Field, Brian T. Chait, and Michael P. Rout Part II Nuclear Envelope Protein Interactions, Localization, and Dynamics4. Super Resolution Microscopy of the Nuclear Envelope and Associated Proteins Wei Xie, Henning F. Horn, and Graham D. Wright Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS) Shimi Takeshi, Chan-Gi Pack, and Robert D. Goldman Probing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP Krishna C. Mudumbi and Weidong Yang The Use of 2-Photon FLIM/FRET to Study Protein Interactions during Nuclear Envelope Fusion In Vivo and In Vitro Richard D. Byrne, Banafshé Larijani, and Dominic L. Poccia Identifying Protein-Protein Associations at the Nuclear Envelope with BioID Dae In Kim, Samuel C. Jensen, and Kyle J. Roux In Situ Detection of Interactions between Nuclear Envelope Proteins and Partners Alice Barateau and Brigitte Buendia Methods for Single-Cell Pulse-Chase Analysis of Nuclear Components Marek Drozdz and David J. Vaux Analysis of Nuclear Lamina Proteins in Myoblast Differentiation by Functional Complementation Olga Tapia and Larry Gerace Analysis of Meiotic Telomere Behavior in the Mouse Jana Link, Ricardo Benavente, and Manfred Alsheimer Part III Nuclear Envelope Interactions with the Cytoskeleton Identification and Validation of Putative Nesprin Variants Flavia Autore, Catherine M. Shanahan, and Qiuping Zhang Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western Blotting James Carthew and Iakowos Karakesisoglou The Use of Polyacrylamide Hydrogels to Study the Effects of Matrix Stiffness on Nuclear Envelope Properties Rose-Marie Minaisah, Susan Cox, and Derek T. Warren Cell Microharpooning to Study Nucleo-Cytoskeletal Coupling Gregory Fedorchak and Jan Lammerding Wound Healing Assays to Study Mechanisms of Nuclear Movement in Fibroblasts and Myoblasts Wakam Chang, Susumu Antoku, and Gregg G. Gundersen Methods for Assessing Nuclear Rotation and Nuclear Positioning in Developing Skeletal Muscle Cells Meredith H. Wilson, Matthew G. Bray, and Erika L. F. Holzbaur Imaging Approaches to Investigate Myonuclear Positioning in Drosophila Mafalda Azavedo, Victoria K. Schulman, Eric Folker, Mridula Balakrishnana, and Mary Baylies Part IV Nuclear Envelope-Chromatin Interactions Mapping Nuclear Lamin-Genome Interactions by Chromatin Immunoprecipitation of Nuclear Lamins Anja R. Oldenburg and Philippe Collas Lamin ChIP from Chromatin Prepared by Micrococcal Nuclease Digestion Isabelle Dubant-Goulet DamID Analysis of Nuclear Organization in Caenorhabditis elegans Georgina Gómez-Saldivar, Peter Meister, and Peter Askjaer The Application of DamID to Identify Peripheral Gene Sequences in Differentiated and Primary Cells Michael I. Robson and Eric C. Schirmer Visualizing the Spatial Relationship of the Genome with the Nuclear Envelope Using Fluorescence in situ Hybridization Craig S. Clements, Ural Bikkul, Mai Hassan Ahmed, Helen A. Foster, Lauren S. Godwin, and Joanna M. Bridger Visualization of Genomic Loci in Living Cells with a Fluorescent CRISPR/Cas9 System Tobias Anton, Heinrich Leonhardt, and Yolanda Markaki Methods to Monitor DNA Repair Defects and Genomic Instability in the Context of a Disrupted Nuclear Lamina Suzanna Gonzalo and Ray Kreienkamp Part V Nucleo-Cytoplasmic Transport High Resolution Scanning Electron Microscopy and Immuno-Gold Labeling of the Nuclear Lamina and Nuclear Pore Complex Martin W. Goldberg An In Vitro Assay to Study Targeting of Membrane Proteins to the Inner Nuclear Membrane Rosemarie Ungricht, Sumit Pawar, and Ulrike Kutay Nuclear Protein Transport in Digitonin Permeabilized Cells Steven A. Adam Analysis of CRM1-Dependent Nuclear Export in Permeabilized Cells Ralph H. Kehlenbach and Sarah A. Port SPEED Microscopy and Its Application in Nucleocytoplasmic Transport Jiong Ma, Joseph M. Kelich, and Weidong Yang


Titel: The Nuclear Envelope
Untertitel: Methods and Protocols
EAN: 9781493935284
ISBN: 1493935283
Format: Fester Einband
Herausgeber: Springer New York
Anzahl Seiten: 540
Gewicht: 1201g
Größe: H260mm x B183mm x T34mm
Jahr: 2016
Auflage: 1st ed. 2016

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