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Telomeres and Telomerase

  • Kartonierter Einband
  • 204 Seiten
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In its updated 2nd Edition, this book builds on the telomerase assays featured in the popular first edition. Using the Methods in ... Weiterlesen
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Beschreibung

In its updated 2nd Edition, this book builds on the telomerase assays featured in the popular first edition. Using the Methods in Molecular Biology(TM) format, it includes materials and reagents lists, step-by-step protocols, and expert tips on troubleshooting.

New and rapid advances in technology have equipped us with a variety of tools and platforms to ask fundamental questions of telomere regulation and have allowed investigators to carry out experiments using diverse model systems. For example, proteomic, genomic, and molecular approaches have afforded us unprecedented insight into the complex protein interaction networks at work on the telomere chromatin and the detailed information regarding telomere dynamics in response to stress or stimuli. Telomeres and Telomerase: Methods and Protocols, Second Edition builds upon the telomerase assays featured in the popular first edition to encompass many different assays that allow investigators to query the function of telomere proteins and the responses of the telomere DNA, including detailed examinations of biochemical, molecular, and proteomic approaches. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Telomeres and Telomerase: Methods and Protocols, Second Edition serves as an ideal, up-to-date guide for investigators further pursing this vital field of study.

Features cutting-edge protocols from this expanding and celebrated field of study

Details biochemical, molecular, and proteomic approaches

Contains expert tips and key implementation advice



Inhalt
1. Introduction to Telomeres and Telomerase Zhou Songyang 2. Analysis of Average Telomere Length in Cultured Human Cells Dan Liu 3. Telomere Length Analysis by Quantitative Fluorescent In situ Hybridization (Q-FISH) Isabelle Ourliac-Garnier and Arturo Londoño-Vallejo 4. Telomere Strand-Specific Length Analysis by Fluorescent In situ Hybridization (Q-CO-FISH) Isabelle Ourliac-Garnier and Arturo Londoño-Vallejo 5. Telomere G-Overhang Length Measurement Method 1: The DSN Method Yong Zhao, Jerry W. Shay, and Woodring E. Wright 6. Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA Hidetoshi Tahara 7. Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-rich Fill-In Yong Zhao, Jerry W. Shay, and Woodring E. Wright 8. G-Quadruplex Structures and G-Quadruplex-Interactive Compounds Raveendra I. Mathad and Danzhou Yang 9. Analysis of Yeast Telomerase by Primer Extension Assays Min Hsu and Neal F. Lue 10. Telomeric Repeat Amplification Protocol (TRAP): Measuring the Activity of the Telomerase Huawei Xin 11. CO-FISH, COD-FISH, ReD-FISH, SKY-FISH Eli S. Williams, Michael N. Cornforth, Edwin H. Goodwin, and Susan M. Bailey 12. Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques Eladio Abreu, Rebecca M. Terns, and Michael P. Terns 13. Cytogenetic Analysis of Telomere Dysfunction Asha Multani and Sandy Chang 14. Probing the Telomere Damage Response Relcha Rai and Sandy Chang 15. Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP) Wenbin Ma 16. Studying of Telomeric Protein-Protein Interactions by Bi-Molecular Fluorescence Complementation (BiFC) and Peptide Array-Based Assays Wenbin Ma, Hyeung Kim, and Zhou Songyang 17. Human Telomere POT1-TPP1 Complex and Its Role in Telomerase Activity Regulation Feng Wang and Ming Lei

Produktinformationen

Titel: Telomeres and Telomerase
Untertitel: Methods and Protocols
Editor:
EAN: 9781493956272
ISBN: 1493956272
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 204
Gewicht: 395g
Größe: H254mm x B178mm x T11mm
Jahr: 2016
Auflage: Softcover reprint of the original 2nd ed. 2011

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