T-Cell Development

This volume provides simple and accessible experiment protocols to explore thymus biology. T-Cell Development: Methods and Protocols is divided into three parts presenting short reviews on T cell development, analysis strategies, protocols for cell preparation, flow cytometry analyses, and multiple aspects of thymocyte biology. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, T-Cell Development: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

200 Million Thymocytes and I: A Beginner's Survival Guide to T cell Development.- Development of gd T Cells, the Special Forces Soldiers of the Immune System.- Genetic Tools to Study T Cell Development.- Assessment of T cell Development by Flow Cytometry.- Flow Cytometry Analysis of Thymic Cells and their Subpopulations.- Identifying the Spatial Relationships of Thymic Stromal and Thymocyte Subsets by Immunofluorescence Analysis.- Purification of Thymocyte and T Cell Subsets.- Retroviral Transduction of T Cells and T Cell Precursors.- Bone Marrow and Fetal Liver Radiation Chimeras.- In vitro Analyses of T Cell Effector Differentiation.- Studying T Cell Development in Thymic Slices.- FTOC-based Analysis of Negative Selection.- Reconstituted Thymus Organ Culture.- Induction of T-cell Development in vitro by Delta-like (Dll)-Expressing Stromal Cells.- In vitro Analysis of Thymocyte Signaling.- Molecular Analysis of Mouse T Cell Receptor a and b Gene Rearrangements.- Intrathymic Injection.- Analysis of Cell Proliferation and |Homeostasis using EdU Labeling.- Characterization and Isolation of Human T Cell Progenitors.- Approaches to Study Human T Cell Development.- Humanized Mice to Study Human T Cell Development.- Using the Zebrafish Model to Study T cell Development.