Stem Cell Migration

In this succinct and practical guide, expert contributors cover standard and novel techniques that facilitate the study of stem cell migration. The book emphasizes in vivo technologies and includes protocols on germ, neuronal, and hematopoietic stem cells.

The migration of stem cells has been found to be critical during early development for the organization of the embryonic body as well as during adult life with tissue homeostasis and regeneration of organ function. Due to the low frequency of these cells in vivo, problems in identifying and prospectively purifying tissue specific stem cells near homogeneity, and, most importantly, a lack of adequate technologies and protocols to study stem cell migration in vivo, this vital research has been quite difficult until recently. In Stem Cell Migration: Methods and Protocols , experts in the field compile and highlight the standard and novel techniques that allow the studying of the migration of stem cells in one succinct manual. Including protocols on germ, neuronal, and hematopoietic stem cells, during development and adulthood with a clear emphasis on in vivo technologies, the volume also extends its coverage to in vitro approaches toward several developmentally-conserved signaling pathways. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and convenient, Stem Cell Migration: Methods and Protocols provides key, state-of the art information on experimental techniques for studying stem cell migration both at a cellular and molecular level in development, regeneration, and disease.

Provides a succinct manual to the cutting-edge techniques in stem cell migration Includes clear and practical step-by-step protocols, perfect for reproducible results Features tips and key implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Trafficking of Stem Cells.- Migratory Strategies of Normal and Malignant Stem Cells.- Hematopoietic Stem Cell Characterization and Isolation.- Isolation and Characterization of Adult Neural Stem Cells.- Magnetic Resonance Imaging of Stem Cell Migration.- Imaging of Schwann Cells In Vivo.- Imaging of Embryonic Stem Cell Migration In Vivo.- In Vivo Germ Line Stem Cell Migration: A Mouse Model.- Live Microscopy of Neural Stem Cell Migration in Brain Slices.- Whole Embryo Imaging of Hematopoietic Cell Emergence and Migration.- Stem Cell Migration: A Zebrafish Model.- Imaging Pluripotent Cell Migration in Drosophila.- Adhesion, Migration, and Homing of Murine Hematopoietic Stem Cells and Progenitors.- Methods to Analyze the Homing Efficiency and Spatial Distribution of Hematopoietic Stem and Progenitor Cells and Their Relationship to the Bone Marrow Endosteum and Vascular Endothelium.- Imaging Hematopoietic Stem Cells in the Marrow of Long Bones In Vivo.- Studies of Adult Neural Stem Cell Migration.- Dissecting Mesenchymal Stem Cell Movement: Migration Assays for Tracing and Deducing Cell Migration.- Epithelial Stem Cells.- Pathways Implicated in Stem Cell Migration: The SDF-1/CXCR4 Axis.- The Role of Receptor Tyrosine Kinases in Primordial Germ Cell Migration.- Rho GTPases in Hematopoietic Stem/Progenitor Cell Migration.