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Stem Cell Migration

  • Kartonierter Einband
  • 340 Seiten
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In this succinct and practical guide, expert contributors cover standard and novel techniques that facilitate the study of stem ce... Weiterlesen
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Beschreibung

In this succinct and practical guide, expert contributors cover standard and novel techniques that facilitate the study of stem cell migration. The book emphasizes in vivo technologies and includes protocols on germ, neuronal, and hematopoietic stem cells.


The migration of stem cells has been found to be critical during early development for the organization of the embryonic body as well as during adult life with tissue homeostasis and regeneration of organ function. Due to the low frequency of these cells in vivo, problems in identifying and prospectively purifying tissue specific stem cells near homogeneity, and, most importantly, a lack of adequate technologies and protocols to study stem cell migration in vivo, this vital research has been quite difficult until recently. In Stem Cell Migration: Methods and Protocols, experts in the field compile and highlight the standard and novel techniques that allow the studying of the migration of stem cells in one succinct manual. Including protocols on germ, neuronal, and hematopoietic stem cells, during development and adulthood with a clear emphasis on in vivo technologies, the volume also extends its coverage to in vitro approaches toward several developmentally-conserved signaling pathways. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and convenient, Stem Cell Migration: Methods and Protocols provides key, state-of the art information on experimental techniques for studying stem cell migration both at a cellular and molecular level in development, regeneration, and disease.



Provides a succinct manual to the cutting-edge techniques in stem cell migration

Includes clear and practical step-by-step protocols, perfect for reproducible results

Features tips and key implementation advice from the experts



Inhalt
Part I: Stem Cells and Migration: An Overview 1. Trafficking of Stem Cells Claire Magnon, Daniel Lucas, and Paul S. Frenette 2. Migratory Strategies of Normal and Malignant Stem Cells David A. Hess and Alison L. Allan Part II: Stem Cells Identification and Microscopic Technologies to Track Transplanted Stem Cells In Vivo 3. Hematopoietic Stem Cell Characterization and Isolation Lara Rossi, Grant A. Challen, Olga Sirin, Karen Kuan-Yin Lin, and Margaret A. Goodell 4. Isolation and Characterization of Adult Neural Stem Cells Florian A. Siebzehnrubl, Vinata Vedam-Mai, Hassan Azari, Brent A. Reynolds, and Loic P. Deleyrolle 5. Magnetic Resonance Imaging of Stem Cell Migration Eva Syková Pavla Jendelová, and Vít Herynek 6. Imaging of Schwann Cells In Vivo Rahul Kasukurthi and Terrence M. Myckatyn 7. Imaging of Embryonic Stem Cell Migration In Vivo Andrew S. Lee and Joseph C. Wu Part III: Stem Cell Migration During Development 8. In Vivo Germ Line Stem Cell Migration: A Mouse Model Brian Dudley and Kathleen Molyneaux 9. Live Microscopy of Neural Stem Cell Migration in Brain Slices Jin-Wu Tsai and Richard B. Vallee 10. Whole Embryo Imaging of Hematopoietic Cell Emergence and Migration Michael J. Ferkowicz and Mervin C. Yoder 11. Stem Cell Migration: A Zebrafish Model Pulin Li and Leonard I. Zon 12. Imaging Pluripotent Cell Migration in Drosophila Michael J. Murray and Robert Saint Part IV: Adult Hematopoietic Stem Cell Migration 13. Adhesion, Migration, and Homing of Murine Hematopoietic Stem Cells and Progenitors Jose A. Cancelas 14. Methods to Analyze the Homing Efficiency and Spatial Distribution of Hematopoietic Stem and Progenitor Cells and Their Relationship to the Bone Marrow Endosteum and Vascular Endothelium Jochen Grassinger and Susan K. Nilsson 15. Imaging Hematopoietic Stem Cells in the Marrow of Long Bones In Vivo Anja Köhler, Hartmut Geiger, and Matthias Gunzer Part V: Non Hematopoietic Stem Cell Migration 16. Studies of Adult Neural Stem Cell Migration Adam C. Puche and Serena Bovetti 17. Dissecting Mesenchymal Stem Cell Movement: Migration Assays for Tracing and Deducing Cell Migration Erika L. Spaeth and Frank Marini 18. Epithelial Stem Cells Kyle M. Draheim and Stephen Lyle Part VI: Pathways Implicated in Stem Cell Migration 19. Pathways Implicated in Stem Cell Migration: The SDF-1/CXCR4 Axis Yaron Vagima, Kfir Lapid, Orit Kollet, Polina Goichberg, Ronen Alon, and Tsvee Lapidot 20. The Role of Receptor Tyrosine Kinases in Primordial Germ Cell Migration Louise Silver-Morse and Willis X. Li 21. Rho GTPases in Hematopoietic Stem/Progenitor Cell Migration Wei Liu, Yuxin Feng, Xun Shang, and Yi Zheng

Produktinformationen

Titel: Stem Cell Migration
Untertitel: Methods and Protocols
Editor:
EAN: 9781493961368
ISBN: 1493961365
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 340
Gewicht: 641g
Größe: H254mm x B178mm x T18mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2011

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