Single Cell Protein Analysis

This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Autorentext
Anup Singh Mailstop 9291 Sandia National Laboratories 7011 East Ave Livermore, CA aksingh@sandia.gov

Inhalt

Single-Cell Western Blotting.- A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. Coli Bacteria.- Enzyme-Linked ImmunoSpot (ELISpot) for Single Cell Analysis.- Photocleavable DNA Barcoding Antibodies for Multiplexed Protein Analysis in Single Cells.- Genome-Wide Analysis of Protein and mRNA Copy Numbers in Single Escherichia Coli Cells with Single-Molecule Sensitivity.- Microfluidic Flow Cytometry for Single Cell Protein Analysis.- Microfluidic Image Cytometry for Single Cell Phenotyping of Human Pluripotent Stem Cells.- Characterizing Phenotypes and Signaling Networks of Single Human Cells by Mass Cytometry.- Multiplex Peptide-MHC Tetramer Staining with Mass Cytometry.- Imaging and Mapping of Tissue Constituents at the Single Cell Level Using MALDI MSI and Quantitative Laser Scanning Cytometry.- SPLIFF: A Single Cell Method to Map Protein-Protein Interactions in Time and Space.- Microfluidic Proximity Ligation Assay for Profiling Signaling Networks with Single-Cell Resolution.- Dynamics and Interactions of Individual Proteins in the Membrane of Single, Living Cells.- Microfluidics Enabled Enzyme Activity Measurement in Single Cells.- Microfluidic Chemical Cytometry for Enzyme Assays of Single Cells.- Quantitative Detection of Nucleocytoplasmic Transport of Native Proteins in Single Cells.