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Signal Transduction Protocols

  • Kartonierter Einband
  • 444 Seiten
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It is increasingly clear that signal transduction is a highly organized and integrated process. Divided into two convenient sectio... Weiterlesen
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It is increasingly clear that signal transduction is a highly organized and integrated process. Divided into two convenient sections, this book focuses on experimental approaches to better understand the complexity of signal transduction.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Part I. Overviews 1. Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling Louis M. Luttrell and Terry P. Kenakin 2. Imaging-based Approaches to Understanding G Protein-Coupled Receptor Signaling Complexes Darlaine Pétrin and Terence E. Hébert 3. Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis John K. Westwick and Jane E. Lamerdin 4. RGS-insensitive Ga Subunits: Probes of G Subtype-selective Signaling and Physiological Functions of RGS Proteins Kuljeet Kaur, Jason M. Kehrl, Raelene A. Charbeneau, and Richard R. Neubig 5. Bioinformatic Approaches to Metabolic Pathways Analysis Stuart Maudsley, Wayne Chadwick, Liyun Wang, Yu Zhou, Bronwen Martin, and Sung Park Part II. Receptor Ligand Interactions 6. Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET Jean-Pierre Vilardaga 7. Using BRET to Detect Ligand-Specific Conformational Changes in Preformed Signaling Complexes Nicolas Audet and Graciela Piñeyro Part III. Receptor Receptor Interactions 8. Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High Density Lipoprotein Particles (rHDL) Gisselle A. Vélez-Ruiz and Roger K. Sunahara 9. Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Hetero-Dimerization Lamia Achour, Maud Kamal, Ralf Jockers, and Stefano Marullo 10. Cell-Surface Protein-Protein Interaction Analysis with Time-Resolved FRET and Snap-tag Technologies: Application to G Protein-Coupled Receptor Oligomerization Laëtitia Comps-Agrar, Damien Maurel, Philippe Rondard, Jean-Philippe Pin, Trinquet Eric, and Laurent Prézeau 11. Analysis of GPCR/Ion Channel Interactions Christophe Altier and Gerald W. Zamponi Part IV. Receptor Effector Coupling 12. Multicolor BiFC Analysis of G Protein Complex Formation and Localization Thomas R. Hynes, Evan A. Yost, Stacy M. Yost, and Catherine H. Berlot 13. Real-Time BRET Assays to Measure G Protein/Effector Interactions Darlaine Pétrin, Mélanie Robitaille, and Terence E. Hébert 14. Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP Brock F. Binkowski, Frank Fan, and Keith V. Wood 15. Simultaneous Real Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters Lianne B. Dale and Stephen S. G. Ferguson Part V. Spatial Control of Signal Transduction 16. Using FRET-based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity Charlene Depry and Jin Zhang 17. Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells Lisa L. Gallegos and Alexandra C. Newton 18. Visualizing Receptor Endocytosis and Trafficking Ali Salahpour and Larry S. Barak 19. Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM Guillermo A. Yudowski and Mark von Zastrow 20. Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy Sudha K. Shenoy Part VI. Protein Protein Interactions 21. Detection and Characterization of Receptor Interactions with PDZ Domains Stefanie L. Ritter and Randy A. Hall 22. Tandem Affinity Purification and Identification of Heterotrimeric G-Protein Associated Proteins Syed M. Ahmed, Avais M. Daulat, and Stéphane Angers 23. Study of G Protein-Coupled Receptor/ßarrestin Interactions within Endosomes Using FRAP Benjamin Aguila, May Simaan, and Stéphane A. Laporte 24. Disrupting Protein Complexes Using Tat-tagged Peptide Mimics Shupeng Li, Sheng Chen, Yu Tian Wang, and Fang Liu 25. Protein-fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic Studies of Protein-Protein Interactions in Living Cells Stephen W. Michnick, Po Hien Ear, Christian Landry, Mohan K. Malleshaiah, and Vincent Messier


Titel: Signal Transduction Protocols
EAN: 9781493961085
ISBN: 149396108X
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 444
Gewicht: 829g
Größe: H254mm x B178mm x T23mm
Jahr: 2016
Untertitel: Englisch
Auflage: Softcover reprint of the original 3rd ed. 2011

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