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Semaphorin Signaling

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This volume arranged into three sections describes biochemical, in vitro, and in vivo protocols on Semaphorins. Chapters focus on ... Weiterlesen
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Beschreibung

This volume arranged into three sections describes biochemical, in vitro, and in vivo protocols on Semaphorins. Chapters focus on approaches that would allow the novice to study Semaphorins and employ robust assays to characterize mechanisms of action. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Semaphorin Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras



Klappentext

This volume arranged into three sections describes biochemical, in vitro, and in vivo protocols on Semaphorins. Chapters focus on approaches that would allow the novice to study Semaphorins and employ robust assays to characterize mechanisms of action. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Semaphorin Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.



Inhalt
1. Semaphorins and their Signaling Mechanisms Laura T. Alto and Jonathan R. Terman Part I: Biochemical Approaches 2. Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells Joanne Soong and Glynis A. Scott 3. Expression and Purification of Class 7 Semaphorin and its Plexinc1 Receptor Using Baculovirus-Mediated Mammalian Cell Gene Transduction Xiaoyan Chen, Po-Han Chen, and Xiaolin He 4. Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System Terukazu Nogi, Emiko Mihara, Norihisa Yasui, and Junichi Takagi 5. Plate-Based Assay for Measuring Direct Semaphorin-Neuropilin Interactions Matthew W. Parker and Craig W. Vander Kooi 6. Characterizing Plexin Gtpase Interactions Using Gel Filtration, Surface Plasmon Resonance Spectrometry and Isothermal Titration Calorimetry Jeannine Muller-Greven, Soonjeung Kim, Prasanta K. Hota, Yufeng Tong, Susmita Borthakur, and Matthias Buck 7. In Vitro Assay for the Rap Gtpase Activating Protein Activity of the Purified Cytoplasmic Domain of Plexin Heath G. Pascoe, Yuxiao Wang, and Xuewu Zhang 8. Characterizing F-Actin Disassembly Induced by the Semaphorin Signaling Component Mical Jimok Yoon, Ruei-Jiun Hung, and Jonathan R. Terman 9. Characterizing Erbb-2-Mediated Tyrosine Phosphorylation and Activation of Plexins Thomas Worzfeld and Jakub M. Swiercz 10. Characterizing PKA-Mediated Phosphorylation of Plexin Using Purified Proteins Taehong Yang and Jonathan R. Terman Part II: In Vitro Approaches 11. Using Heterologous Cos-7 Cells to Identify Semaphorin Signaling Components Atsuko Sakurai, Colleen L. Doçi, and J. Silvio Gutkind 12. Analysis of Semaphorin-Induced Growth Cone Collapse and Axon Growth Inhibition Lionel Meyer, Andrius Kaselis, Saulius Satkauskas, and Dominique Bagnard 13. Using Rotary Shadow Electron Microscopy to Characterize Semaphorin-Mediated Growth Cone Collapse Paul C. Bridgman 14. An Electrical Impedance-Based Method for Quantitative Real-Time Analysis of Semaphorin-Elicited Endothelial Cell Collapse Chiara Camillo, Noemi Gioelli, Federico Bussolin, and Guido Serini 15. Regulation of Cortical Dendrite Morphology and Spine Organization by Secreted Semaphorins A Primary Culture Approach Sheng-Shiang Peng and Tracy S. Tran 16. Characterizing Semaphorin Signaling Using Isolated Neurons in Culture Sophie Chauvet, Erik Mire, and Fanny Mann 17. Performing Axon Orientation Assays With Secreted Semaphorins And Other Guidance Cues Céline Delloye-Bourgeois, Frédéric Moret And Valérie Castellani 18. Assays to Examine Transmembrane Semaphorin Function In Vitro Rachel Q. Liu, Kristen Browne, and Timothy P. O'Connor 19. Micro-CALI to Study Localized Roles of the Semaphorin Signaling Component CRMP in Axon Growth Yoshio Goshima and Kohtaro Takei 20. Visualizing and Characterizing Semaphorin Endocytic Events Using Quantum Dot Conjugated Proteins Ioana Carcea and Deanna L. Benson 21. Visualization of Clathrin-Mediated Endocytosis during Semaphorin-Guided Axonal Growth Rurika Itofusa, Takuro Tojima, and Hiroyuki Kamiguchi 22. Tracking Differential Endocytosis and Trafficking of Semaphorin Receptor Complexes in Responding Nerve Growth Cones Puneet Dang and Andrew J. W. Furley 23. Antibody-Feeding Assay: A Method to Track the Internalization of Neuropilin-1 and Other Cell Surface Receptors Sabrina Rizzolio and Luca Tamagnone 24. Photolithography-Based Substrate Microfabrication for Patterning Semaphorin 3A to Study Neuronal Development Maya Shelly, Seong-Il Lee, Giulia Suarato, Yizhi Meng, and Sophie Pautot 25. Characterization of Semaphorin 6A-Mediated Effects on Angiogenesis through Regulation of VEGF Signaling Hidetaka Ohnuki and Giovanna Tosato 26. Studying the Effects of Semaphorins on Oligodendrocyte Lineage Cells Yasir Ahmed Syed Sarah Ali Abdulla, and Mark. R. N. Kotter 27. Characterizing Semaphorin-Mediated Immune Responses Using an Antigen-Presentation Assay Satoshi Nojima and Atsushi Kumanogoh 28. Podocyte Shape Regulation by Semaphorin 3A and MICAL-1 Alda Tufro Part III: In Vivo Approaches 29. In Vivo and In Vitro Knockdown Approaches in the Avian Embryo as a Means to Study Semaphorin Signaling Karli Montague, Sarah Guthrie, and Ivana Poparic 30. Assaying the Action of Secreted Semaphorins on Vascular Permeability Lucas Treps and Julie Gavard 31. Characterization of the Effects of Semaphorin 4D Signaling on Angiogenesis Hua Zhou, Ying-Hua Yang, and John R. Basile 32. Characterizing Semaphorin-Mediated Effects on Sensory and Motor Axon Pathfinding and Connectivity during Embryonic Development Rosa Eva Huettl and Andrea B. Huber 33. Experimental Approaches for Studying Semaphorin Signals in Tumor Growth and Metastasis in Mouse Models Enrico Moiso, Massimo Accardo, and Luca Tamagnone 34. Characterizing Semaphorin Signaling In Vivo Using C. Elegans Akira Nukazuka and Shin Takagi

Produktinformationen

Titel: Semaphorin Signaling
Untertitel: Methods and Protocols
Editor:
EAN: 9781493964468
ISBN: 978-1-4939-6446-8
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 503
Gewicht: 1094g
Größe: H35mm x B261mm x T187mm
Jahr: 2016
Untertitel: Englisch
Auflage: 1st ed. 2017

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