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Salmonella: Methods and Protocols presents detailed methods on a variety of aspects of Salmonella research, focusing on those whic... Weiterlesen
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Salmonella: Methods and Protocols presents detailed methods on a variety of aspects of Salmonella research, focusing on those which provide landmarks for future discovery. Highlighted topics include molecular genotyping, rapid and accurate new methods for sensitive determination of antibiotic resistance profiles, the characterization of Salmonella-specific cell antigens, and Salmonella-host cell interactions. Detailed methods for studying Salmonella motility are also presented. Each chapter provides a short overview of the topic, which is followed by detailed explanations of techniques such as genetic manipulation and molecular imaging that offer the possibilities of using Salmonella as a therapeutic vector to interfere with disease. This is the first comprehensive volume of methods and protocols for studying Salmonella and will be indispensable to researchers engaged in the study of Salmonella, and enterobacteria in general. Because no recent comprehensive literature of this format is available on Salmonella, Salmonella: Methods and Protocols will be of value to a wide variety of researchers engaged in the study of Salmonella, and enterobacteria in general.

The first comprehensive collection of Salmonella methods and protocols

Explains basic techniques that can be easily applied to any enterobacteria

Each chapter includes a short overview followed by detailed explanations of techniques

Table of Contents 1. Quantitative, Multiplexed Detection of Salmonella and Other Pathogens by Luminex Sherry A. Dunbar and James W. Jacobsen 2. Detection of Salmonella by Bacteriophage Felix 01 Jonathan C. Kuhn 3. Physical Mapping of Salmonella Genomes Shu-Lin Liu 4. Cloth-based Hybridization Array System for the Identification of Antibiotic Resistance Genes in Salmonella Burton W. Blais and Martine Gauthier 5. Genome-based Identification of Molecular Analyses of Pathogenicity Islands and Genomic Islands in Salmonella enterica Michael Hensel 6. Determination of the Gene Content of Salmonella Genomes by Microarray Analysis Steffen Porwollik and Michael McClelland 7. In-Vivo Excision, Cloning, and Broad-Host-Range Transfer of Large Bacterial DNA Segments Using VEX-Capture James A. Wilson and Cheryl A. Nickerson 8. Amplified Fragment Length Polymorphism Analysis of Salmonella enterica Ruiting Lan and Peter R. Reeves 9. Salmonella Phages and Prophages - Genomics and Practical Aspects Andrew M. Kropinski, Alexander Sulakvelidze, Paulina Konczy, and Cornelius Poppe 10. Salmonella Typhimurium Phage Typing for Pathogens Wolfgang Rabsch 11. Salmonella Phages Examined in the Electron Microscope Hans-W. Ackermann 12. Applications of Cell Imaging in Salmonella Research Charlotte A. Perrett and Mark A. Jepson 13. Analysis of Kinesin Accumulation on Salmonella-containing Vacuoles Audrey Dumont, Nina Schroeder, Jean-Pierre Gorvel, and Stéphane Méresse 14. Magnesium, Manganese and Divalent Cation Transport Assays in Intact Cells Michael E. Maguire 15. Methods in Cell-to-Cell Signaling in Salmonella Brian M. M. Ahmer, Jenee N. Smith, Jessica L. Dyszel, and AmberLindsay 16. Development of Salmonella Strains as Cancer Therapy Agents and Testing in Tumor Cell Lines AbrahamEisenstark, Robert A. Kazmierczak, Alison Fea, Rula Khreis, Dustin Newman, and Heide Schatten 17. Further Resources for Molecular Protocols in Salmonella Research Abraham Eisenstark and Kelly K. Edwards


Titel: Salmonella
Untertitel: Methods and Protocols
EAN: 9781617376740
ISBN: 978-1-61737-674-0
Format: Kartonierter Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 364
Größe: H229mm x B229mm x T152mm
Jahr: 2010
Untertitel: Englisch
Auflage: Softcover reprint of hardcover

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