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RNAi and Plant Gene Function Analysis

  • Kartonierter Einband
  • 256 Seiten
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With RNAi technology now essential to most plant science researchers, this book introduces the central topics, lists the necessary... Weiterlesen
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Beschreibung

With RNAi technology now essential to most plant science researchers, this book introduces the central topics, lists the necessary materials and reagents, offers reproducible laboratory protocols, and provides notes on troubleshooting and avoiding pitfalls.


The use of RNAi technology is essential for most plant science researchers. As DNA sequence information increases, so the need for functional annotation of target genes also increases. Authoritative and accessible, RNAi and Plant Gene Function Analysis: Methods and Protocols seeks to serve as an ideal guide to scientists of all backgrounds looking to further their knowledge of this fundamental science. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

In addition to the functional annotation of unknown genes, RNAi technology has been applied to the genetic engineering of important plant metabolites including starches, oils, and storage proteins. RNAi has been used to engineer plants resistant to plant viruses and also to nematodes and insects. RNAi and Plant Gene Function Analysis: Methods and Protocols will provide both professionals and novices of plant biotechnology and breeding research with essential tips for designing experiments meant to explore plant gene function.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts



Zusammenfassung
RNAi and Plant Gene Function Analysis: Methods and Protocols will provide both professionals and novices of plant biotechnology and breeding research with essential tips for designing experiments meant to explore plant gene function.

Inhalt

1. Overview of Plant RNAi

Yuichiro Watanabe

2. Caveat of RNAi in Plants: the Off-Target Effect

Muthappa Senthil-Kumar and Kirankumar S. Mysore

3. Plant Gateway Vectors for RNAi as a Tool for Functional Genomic Studies

Toshiya Muranaka

4. Heat-Inducible RNAi for Gene Functional Analysis in Plants

Frédéric Masclaux and Jean-Philippe Galaud

5. Gene Function Analysis by Artificial MicroRNAs in Physcomitrella patens

Basel Khraiwesh, Isam Fattash, M. Asif Arif, and Wolfgang Frank

6. Virus-Induced Gene Silencing in Ornamental Plants

Cai-Zhong Jiang, Jen-Chih Chen, and Michael Reid

7. Local RNA Silencing Mediated by Agroinfiltration

Jutta Maria Helm, Elena Dadami, and Kriton Kalantidis

8. Direct Transfer of Synthetic Double-Stranded RNA into Protoplasts of Arabidopsis thaliana

Ha-il Jung, Zhiyang Zhai and Olena K. Vatamaniuk

9. Detection of Long and Short Double-Stranded RNAs

Toshiyuki Fukuhara, Syunichi Urayama, Ryo Okada, Eri Kiyota, and Hiromitsu Moriyama

10. Quantitative Stem-Loop RT-PCR for Detection of MicroRNAs

Erika Varkonyi-Gasic and Roger P. Hellens

11. Large Scale Sequencing of Plant Small RNAs

William P. Donovan, Yuanji Zhang, and Miya D. Howell

12. Computational Prediction of Plant miRNA Targets

Ying-Hsuan Sun, Shanfa Lu, Rui Shi, and Vincent L. Chiang

13. Bisulfite S equencing for C ytosine- M ethylation A nalysis in Plants

Nazmul Haque and Masamichi Nishiguchi

14. Using Nuclear Run-On Transcription Assays in RNAi Studies

Basel Khraiwesh

15. Proteomic Analysis of RNA-Interference Induced Knockdown Plant

Sang Yeol Lee and Kyun Oh Lee

16. Comparative Analysis of Phosphoprotein Expression using 2D-DIGE

Tomoya Asano and Takumi Nishiuchi

Produktinformationen

Titel: RNAi and Plant Gene Function Analysis
Untertitel: Methods and Protocols
Editor:
EAN: 9781627039321
ISBN: 1627039325
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 256
Gewicht: 542g
Größe: H260mm x B193mm x T13mm
Jahr: 2014
Auflage: 2011

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