RNA Interference

The field of RNA interference has grown at a breakneck pace. In RNA Interference: From Biology to Clinical Applications, expert contributors provide a refreshing overview of the current science and protocols that span the biological disciplines of this field.

From the early days when RNA interference was a strange artifact in worms to the 2006 Noble Prize received by Fire and Mello and the current clinical trials, the field of RNA interference has grown at a breakneck pace. In RNA Interference: From Biology to Clinical Applications , expert contributors provide an overview of the most current science and protocols that span the biological disciplines from detailed nucleic acid chemistry, to pharmacology, to the manipulation of signal transduction pathways. Divided into three distinct sections, this volume delves into the physiology of RNA interference, RNA interference in the laboratory and siRNA delivery, and preclinical and clinical issues associated with the use of RNAi-inducing agents as drugs in order to stimulate new questions and offer the tools necessary to start addressing those questions. Written in the highly successful Methods in Molecular Biology ™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and inspiring, RNA Interference: From Biology to Clinical Applications aims to promote and motivate innovation by reviewing what has been done, providing details of how it has been done, and encouraging speculation on what the future may hold.

Provides a global overview of the state-of-the-art in RNA interference and practical details used experimentally in modern day experiments Deciphers, organizes, and explains the myriad of techniques used in gene silencing research Stimulates new experimental possibilities by gathering approaches that generally would not be present in the same volume Includes supplementary material: sn.pub/extras

Inhalt
Physiology of RNA Interference.- Endogenous Antiviral Mechanisms of RNA Interference: A Comparative Biology Perspective.- Monitoring Innate Immune Recruitment by siRNAs in Mammalian Cells.- Current Knowledge of MicroRNAs and Noncoding RNAs in Virus-Infected Cells.- Allele-Specific Silencing by RNA Interference.- Computational siRNA Design Considering Alternative Splicing.- Bioinformatic Approaches to siRNA Selection and Optimization.- Optimized Gene Silencing by Co-expression of Multiple shRNAs in a Single Vector.- Strategies in Designing Multigene Expression Units to Downregulate HIV-1.- Designing Optimal siRNA Based on Target Site Accessibility.- Chemical Synthesis of 2?-O-Alkylated siRNAs.- RNA Interference in the Laboratory and siRNA Delivery.- siRNA Specific Delivery System for Targeting Dendritic Cells.- Hydrodynamic Delivery Protocols.- New Methods for Reverse Transfection with siRNA from a Solid Surface.- Nonviral siRNA Delivery for Gene Silencing in Neurodegenerative Diseases.- Using siRNA to Uncover Novel Oncogenic Signaling Pathways.- Biodegradable Polymer-Mediated sh/siRNA Delivery for Cancer Studies.- Cellular siRNA Delivery Using TatU1A and Photo-Induced RNA Interference.- Polyethylenimine (PEI)/siRNA-Mediated Gene Knockdown In Vitro and In Vivo.- Transfection of siRNAs in Multiple Myeloma Cell Lines.- Clinical Implementation.- A New Approach for Therapeutic Use by RNA Interference in the Brain.- Inhibitory RNA Molecules in Immunotherapy for Cancer.- Preventing Tissue Injury Using siRNA.- Preventing Immune Rejection Through Gene Silencing.- Topical Application of siRNA Targeting Cutaneous Dendritic Cells in Allergic Skin Disease.- Direct Application of siRNA for In Vivo Pain Research.- A Potential Therapeutic for Pandemic Influenza Using RNAInterference.- Evaluation of Targets for Ovarian Cancer Gene Silencing Therapy: In Vitro and In Vivo Approaches.