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RNA Imaging

  • Kartonierter Einband
  • 232 Seiten
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This detailed volume will focus on the phenomenon of RNA interference by providing comprehensive coverage of various techniques fo... Weiterlesen
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This detailed volume will focus on the phenomenon of RNA interference by providing comprehensive coverage of various techniques for in vivo micro/siRNA imaging including the design and synthesis of specific imaging agents and tools, the development of imaging methodologies, and their interpretation. An essential element in the development and optimization of these therapies is the ability to measure the bioavailability and functionality of the RNA/oligonucleotide molecule after administration into the body. Noninvasive imaging provides the necessary set of tools to accomplish this in authentic physiologic environments and across time. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, RNA Imaging: Methods and Protocols serves physicians, scientists, and graduate students who are either new to the field of RNA-based imaging and its associated therapeutic applications or who wish to be apprised of recent advances in the state of the art.

Includes cutting-edge methods and protocols for the study of RNA imaging

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts


1. Imaging Functional Nucleic Acid Delivery to Skin

Roger L. Kaspar, Robyn P. Hickerson, Emilio González-González, Manuel A. Flores, Tycho P. Speaker, Faye A. Rogers, Leonard M. Milstone, and Christopher H. Contag

2. In Vivo Magnetic Resonance Imaging of Small Interfering RNA Nanodelivery to Pancreatic Islets

Ping Wang and Anna Moore

3. Magnetic Resonance Spectroscopy of siRNA-Based Cancer Therapy

Marie-France Penet, Zhihang Chen, Noriko Mori, Balaji Krishnamachary, and Zaver M. Bhujwalla

4. Targeted Delivery with Imaging Assessment of siRNA Expressing Nanocassettes into Cancer

Wei Chen and Lily Yang

5. Analyses of Tumor Burden In Vivo and Metastasis Ex Vivo Using Luciferase-Expressing Cancer Cells in an Orthotopic Mouse Model of Neuroblastoma

Frances L. Byrne, Joshua A. McCarroll, and Maria Kavallaris

6. Indium-Labeling of siRNA for Small Animal SPECT Imaging

Steven Jones and Olivia Merkel

7. Imaging of Electrotransferred siRNA

Muriel Golzio and Justin Teissié

8. Whole-Body Scanning PCR, a Tool for the Visualization of the In Vivo Biodistribution Pattern of Endogenous and Exogenous Oligonucleotides in Rodents

Julien A. Boos and Iwan Beuvink

9. siRNA Nanoparticles for Ultra-Long Gene Silencing In Vivo

Seung Koo Lee

and Ching-Hsuan Tung

10. Sensing miRNA: Signal Amplification by Cognate RISC for Intracellular Detection of miRNA in Live Cells

Amol Kavishwar and Zdravka Medarova

11. Molecular Beacon-Based MicroRNA Imaging During Neurogenesis

Jonghwan Lee and Soonhag Kim

12. Hypoxia-Responsive Copolymer for siRNA Delivery

Federico Perche, Swati Biswas, Niravkumar R. Patel, and Vladimir P. Torchilin

13. Controlling RNA Expression in Cancer Using Iron Oxide Nanoparticles Detectable by MRI and In Vivo Optical Imaging

Zdravka Medarova, Mustafa Balcioglu, and Mehmet V. Yigit

14. Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures

Karmele Valencia and Fernando Lecanda

15. Positive Bioluminescence Monitoring of MicroRNA Expression in Small Animal Models Using an Engineered Genetic-Switch Expression System, RILES

Patrick Baril and Chantal Pichon

16. MicroRNA Imaging in Combination with Diagnostic Ultrasound and Bubble Liposomes for MicroRNA Delivery

Yoko Endo-Takahashi, Yoichi Negishi, Ryo Suzuki, Kazuo Maruyama, and Yukihiko Aramaki


Titel: RNA Imaging
Untertitel: Methods and Protocols
EAN: 9781493949748
ISBN: 1493949748
Format: Kartonierter Einband
Herausgeber: Springer New York
Genre: Medizin
Anzahl Seiten: 232
Gewicht: 445g
Größe: H254mm x B178mm x T12mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2016

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