Insilico homology modelling of Papaya ring spot virus coat protein

In this study, coat-protein sequence was analyzed and modelled to explore properties and structure Papaya leaf curl virus, Papaya Mosaic virus, Papaya ring spot virus-P & W.Since no structural information is available for majority of protein sequences a vailable in Protein Data Bank, computational methods for protein structure prediction have been of much interest in recent years. Physico-chemical properties of the protein under study were computed. The grand average ofhydropathy (GRAVY) value,pI, Extinction Coefficient, Aliphatic Index and instability index were found for all four strain of Papaya virus. Its supporting the protein to have better interaction with water. Homology modelling was performed withPHYRE2, ModWeband Swiss Model tool. The model was validated using PROSESS software tool which resulting ramachandran plot for Papaya virus strain. The study may help in understanding the protein function, number and types of epitopes, immunogenic portions, andsuitability for antibody production, taxonomic studies,evaluation studies and virus diagnostics.

Autorentext

O Dr. RISHEE K. KALARIA concluiu o seu doutoramento em biologia molecular e biotecnologia vegetal na Universidade Agrícola de Navsari em 2014. Atualmente, trabalha como Professor Assistente no Instituto de Biotecnologia Aspee Shakilam, Universidade Agrícola de Navsari. É também responsável pela secção de bioinformática.