Recombinant Proteins From Plants

This text sees expert researchers explore plants and their potential for the production of increasingly safe, high quality and biologically active complex recombinant pharmaceutical proteins. Chapters present several production platforms.

Altogether, the biochemical, technical and economic limitations on existing proka- otic and eukaryotic expression systems and the growing clinical demand for complex therapeutic proteins have created substantial interest in developing new expression systems for the production of therapeutic proteins. To that end, plants have emerged in the past decade as a suitable alternative to the current production systems, and today their potential for production of high quality, much safer and biologically active complex recombinant pharmaceutical proteins is largely documented. The chapters in this volume, contributed by leaders in the field, sum up the state-- the-art methods for using a variety of different plants as expression hosts for phar- ceutical proteins. Several production platforms are presented, ranging from seed- and leaf-based production in stable transgenic plant lines, to plant cell bioreactors, to viral or Agrobacterium-mediated transient expr ession systems. Currently, antibodies and their derived fragments represent the largest and most important group of biote- nological products in clinical trials. This explains why the potential of most prod- tion platforms is illustrated here principally for antibodies or antibody fragments with acknowledged potential for immunotherapy in humans. In addition, a comparison of different plant expression systems is presented using aprotinin, a commercial phar- ceutical protein, as a test system. Although multiple books and monographs have been recently published on mol- ular pharming, there is a noticeable dearth of bench step-by-step protocols that can be used quickly and easily by beginners entering this new field.

Fully up-to-date edition offering cutting edge methods for production of recombinant proteins in plants Provides readily reproducible protocols for researchers in the field of molecular pharming Includes supplementary material: sn.pub/extras

Klappentext

The past decade has seen the emergence of plants as a viable alternative to the current production systems for therapeutic proteins. In Recombinant Proteins from Plants, expert researchers explore plants and their potential for the production of increasingly safe, high quality and biologically active complex recombinant pharmaceutical proteins. Chapters present several production platforms, focusing on the largest and most important group of biotechnological products in clinical trials: antibodies and their derived fragments with acknowledged potential for immunotherapy in humans. Due to the shortage of step by step protocols that can be used easily by beginners, this volume aims to fill the void with several chapters of detailed instructions for using the main plant expression systems. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Recombinant Proteins from Plants serves as an ideal guide for those new and seasoned plant scientists, pharmaceutical scientists, and molecular and cellular biologists interested in molecular pharming.

Inhalt
From Neanderthal to Nanobiotech: From Plant Potions to Pharming with Plant Factories.- Cowpea Mosaic Virus-Based Systems for the Expression of Antigens and Antibodies in Plants.- Transient Expression of Antibodies in Plants Using Syringe Agroinfiltration.- Rapid System for Evaluating Bioproduction Capacity of Complex Pharmaceutical Proteins in Plants.- Production and Localization of Recombinant Pharmaceuticals in Transgenic Seeds.- Production of Antibody Fragments in Arabidopsis Seeds.- Production of Plantibodies in Nicotiana Plants.- Physcomitrella patens : A Non-Vascular Plant for Recombinant Protein Production.- Production of Recombinant Proteins in SuspensionCultured Plant Cells.- Chloroplast-Derived Vaccine Antigens and Biopharmaceuticals: Protocols for Expression, Purification, or Oral Delivery and Functional Evaluation.- Protein Body Induction: A New Tool to Produce and Recover Recombinant Proteins in Plants.- A Case Study for Plant-Made Pharmaceuticals Comparing Different Plant Expression and Production Systems.- Glycosylation of Antibody Therapeutics: Optimisation for Purpose.- N-Glycosylation of Plant Recombinant Pharmaceuticals.- Companion Protease Inhibitors to Protect Recombinant Proteins in Transgenic Plant Extracts.- Strategies for Improving Vaccine Antigens Expression in Transgenic Plants: Fusion to Carrier Sequences.- Immunomodulation of Plant Function by In Vitro Selected Single-Chain Fv Intrabodies.- On-Chip Detection of Low-Molecular-Weight Recombinant Proteins in Plant Crude Extracts by SELDI-TOF MS.- Assessing the Risk of Undesirable Immunogenicity/ Allergenicity of Plant-Derived Therapeutic Proteins.- Biosafety, Risk Assessment and Regulation of Plant-Made Pharmaceuticals.