Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition

This volume provides a detailed step-by-step description of protocols for the establishment of in vitro cultures of important medicinal plants, their mass multiplication in controlled environment, and step-wise secondary metabolite analysis. Many of these protocols also provide a basis for in vitro germplasm conservation or cryopreservation of medicinal plant species. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Protocols for In Vitro Mass Multiplication and Analysis of Medicinally Important Phenolics of a Salep Orchid, Satyrium nepalense D.Don ("Salam Mishri").- In vitro culture and phytochemical analysis of Passiflora tenuifila Killip and Passiflora setacea DC (Passifloraceae).- Shoot Tip Meristem Cryopreservation of H ypericum Species.- Protocols for Biotechnological Interventions in Improvement of Vanilla ( Vanilla planifolia Andrews.).- Assessment of In Vitro Biological Activities Of Rich-anthocyanins Plant Species Based on Plinia cauliflora Study Model.- Biotechnological Approaches for Biomass and Cardenolides Production in Digitalis purpurea L..- In vitro regeneration of endangered medicinal plant Heliotropium kotschyi (Ramram).- Micropropagation and biomass production of true-to-type Stevia rebaudiana Bertoni.- Panax ginseng Adventitious Root Suspension Culture: Protocol for Biomass Production and Analysis of Ginsenosides by High Pressure Liquid Chromatography.- Immobilization of Rubia tinctorum L. Suspension Cultures and Biomass Production.- In Vitro Propagation and Conservation of Bacopa monnieri L..- Establishment, Culture and Scale-Up ofBrugmansia candida Hairy Roots for the Production Of Tropane Alkaloids.- Micropropagation, Acclimatization, and Greenhouse Culture of Veratrum californ icum.- In vitro propagation of Withania somnifera (L.) Dunal.- In vitro propagation ofSambong ( Blumea balsamifera Linn.).- Production of Gymnemic Acid From Cell Suspension Cultures of Gymnema sylvestre.- Scale up of Agrobacterium Rhizogenes Mediated Hairy Root Cultures of Rauwolfia serpentina : A persuasive Approach for Stable Reserpine Production.- In vitro shoot cultures and analysis of steroidal lactones in Withania coagulans (Stocks) Dunal.- In vitro Propagation of Cannabis sativa L. and Evaluation of Regenerated Plants for Genetic Fidelity and Cannabinoids Content for the Quality Assurance.-Transcript Quantification of Genes Involved in Steviol Glycoside Biosynthesis in Stevia rebaudiana Bertoni by Real Time Polymerase Chain Reaction (RT-PCR).- In Vitro Propagation and Conservation of Withania somnifera (Dunal) L..- Construction of Hypericin Glands Specific cDNA Library via Suppression Subtractive Hybridization.- In Vitro and Cryopreservation Techniques for Conservation of Snow Mountain Garlic.- In Vitro Callus Induction and Plant Regeneration from Stem Explants of Ceropegia noorjahaniae ; A Critically Endangered Medicinal Herb.- Somatic Embryogenesis of Date Palm ( Phoenix dactylifera L.) through Cell Suspension Culture.- Protocols for Improvement Of Black Pepper Utilizing Biotechnological Tools.- Protocols for In Vitro Propagation, Conservation, Synthetic Seed Production, Microrhizome Production and Molecular Profiling in Turmeric ( Curcuma longa L.).- In Vitro Propagation, and Genetic TransformationIn Ginger ( Zingiber officinale Roscoe.).- Hairy Root Cultures of Gymnema sylvestre R. Br. to Produce Gymnemic Acid.- Bioreactors for Mass Propagation of Cymbopogon citratus Sapf. .