This volume discusses the latest mass spectrometry (MS)-based technologies for proteoform identification, characterization, and quantification. Some of the topics covered in this book include sample preparation, proteoform separation, proteoform gas-phase fragmentation, and bioinformatics tools for MS data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Proteoform Identification: Methods and Protocols is a valuable resource for researchers in both academia and the biopharmaceutical industry who are interested in proteoform analysis using MS.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Proteoforms and Proteoform Families: Past, Present, and Future.- Membrane Ultrafiltration-Based Sample Preparation Method and Sheath-Flow CZE-MS/MS for Top-Down Proteomics.- Size Exclusion Chromatography Strategies and MASH Explorer for Large Proteoform Characterization.- RPLC-RPLC-MS/MS for Proteoform Identification.- Monolithic Materials-Based RPLC-MS for Proteoform Separation and Identification.- Capillary Isoelectric Focusing: Mass Spectrometry Method for the Separation and Online Characterization of Monoclonal Antibody Charge Variants at Intact and Subunit Level.- Proteoform Analysis and Construction of Proteoform Families in Proteoform Suite.- Top-Down Mass Spectrometry Data Analysis Using TopPIC Suite.- Accurate Proteoform Identification and Quantitation Using pTop 2.0.- Proteoform Identification and Quantification using Intact Protein Database Search Engine ProteinGoggle.- Mass Deconvolution of Top-Down Mass Spectrometry Datasets by FLASHDeconv.- Deconvolving Native and Intact Protein Mass Spectra with UniDec.- Discovery of Unknown Post-Translational Modifications by Top-Down Mass Spectrometry.- Determining Copper and Zinc Content in Superoxide Dismutase using Electron Capture Dissociation under Native Spray Conditions.- Surface-Induced Dissociation for Protein Complex Characterization.