Protein Scaffolds

This volume provides a wide range of methods and protocols detailing various protein structures as platforms for building architectures with targeted application. Chapters guide the readers through exploiting a number of protein scaffolds including virus nanoparticles, elastin and collagen peptides and proteins, and other protein templates for either building materials or presentation of ligands. Site-specific bioconjugation methods, some unique protein architectures, and techniques that exploit peptide amphiphile micelles and assembly of chaperones are also featured. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Protein Scaffolds: Design, Synthesis, andApplications aims to ensure successful results in the study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Nanoreactors via Encapsulation of Catalytic Gold Nanoparticles within Cowpea Chlorotic Mottle Virus.- Encapsulation of Active Enzymes within Bacteriophage P22 virus-like Particles.- Encapsulation of Exogenous Proteins in Vault Nanoparticles.- Enzyme Encapsulation in an Engineered Lumazine Synthase Protein Cage.- Metal Ion-induced Self-Assembly and Packaging of CCMV Nanocapsules.- Modification of CCMV Nanocages for Enzyme Encapsulation.- Synthesis of Cross-linked 2-aminoethyl Methacrylate in P22 Viral Capsid via Atom-transfer Radical Polymerization.- Synthesis of Metal-organic Frameworks on Tobacco Mosaic Virus Templates.- TMV Disk Scaffolds for Making sub-30 nm Silver Nanorings.- TMV Disk Scaffolds for Making sub-30 nm Silver Nanorings.- Synthesis of Triangular Silver and Gold Nanoprisms Using Consensus Sequence Tetratricopeptide Repeat Proteins.- Type III Secretion Filaments as Templates for Metallic Nanostructure Synthesis.- Incorporation of Non-canonical Amino Acids into Proteinsby Global Reassignment of Sense Codons.- Modification of Protein Scaffolds via Copper-catalyzed AzideAlkyne Cycloaddition.- Antibody Modification of p-aminophenylalanine-containing Proteins.- Cross-linked Collagen Gels using Gold Nanoparticles.- Genipin Cross-linking Of Elastin and Elastin-based Proteins.- Characterization of Self-Assembled Protein Scaffolds from Collagen-mimetic Peptides.- Design and Characterization of Fibers and Bionanocomposites Using the Coiled-coil Domain of Cartilage Oligomeric Matrix Protein.- Preparing Genes for Repetitive Elastin-like Polypeptides Using Gibson Assembly.- Peptide Amphiphile Micelles for Vaccine Delivery.- Controlled Assembly of the Filamentous Chaperone Gamma-prefoldin into Defined Nanostructures.- Advances in the Application of Designed Ankyrin Repeat Proteins (Darpins) As Research Tools and Protein Therapeutics.