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Protein Microarray for Disease Analysis

  • Kartonierter Einband
  • 388 Seiten
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This comprehensive survey of the field sees expert researchers provide concise descriptions of the most up-to-date methodologies u... Weiterlesen
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This comprehensive survey of the field sees expert researchers provide concise descriptions of the most up-to-date methodologies used to fabricate microarrays for the analysis of proteins or responses to proteins that help inform our study of human disease.

Protein microarrays have been used for a wide variety of important tasks, such as identifying protein-protein interactions, discovering disease biomarkers, identifying DNA-binding specificity by protein variants, and for characterization of the humoral immune response. In Protein Microarray for Disease Analysis: Methods and Protocols, expert researchers provide concise descriptions of the methodologies currently used to fabricate microarrays for the comprehensive analysis of proteins or responses to proteins that can be used to dissect human disease. These methodologies are the toolbox for revolutionizing drug development and cell-level biochemical understanding of human disease processes. Beginning with a section on protein-detecting analytical microarrays, the volume continues with sections covering antigen microarrays for immunoprofiling, protein function microarrays, the validation of candidate targets, proteomic libraries, as well as signal detection strategies and data analysis techniques. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Protein Microarray for Disease Analysis: Methods and Protocols serves as a solid framework to aid scientists in understanding how protein microarray technology is presently developing and how it can be applied to transform our analysis of human disease.

Serves as a toolbox of methodologies currently used in the field of protein microarray study

Features step-by-step protocols ready for the lab

Contains expert tips and vital implementation advice to ensure success

Part I: Protein-Detecting Analytical Microarrays 1. Detecting and Quantifying Multiple Proteins in Clinical Samples in High-Throughput Using Antibody Microarrays Tanya Knickerbocker and Gavin MacBeath 2. Analysis of Serum Protein Glycosylation with Antibody-Lectin Microarray for High-Throughput Biomarker Screening Chen Li and David M. Lubman 3. Antibody Suspension Bead Arrays Jochen M. Schwenk and Peter Nilsson 4. Reverse Protein Arrays Applied to Host-Pathogen Interaction Studies Víctor J. Cid, Ekkehard Kauffmann, and María Molina 5. Identification and Optimization of DNA Aptamer Binding Regions Using DNA Microarrays Nicholas O. Fischer and Theodore M. Tarasow 6. Recombinant Lectin Microarrays for Glycomic Analysis Daniel C. Propheter, Ku-Lung Hsu, and Lara K. Mahal Part II: Antigen Microarrays for Immunoprofiling 7. Recombinant Antigen Microarrays for Serum/Plasma Antibody Detection Persis P. Wadia, Bita Sahaf, and David B. Miklos 8. SPOT Synthesis as a Tool to Study Protein-Protein Interactions Dirk F.H. Winkler, Heiko Andresen, and Kai Hilpert 9. Native Antigen Fractionation Protein Microarrays for Biomarker Discovery Robert J. Caiazzo, Jr., Dennis J. O'Rourke, Timothy J. Barder, Bryce P. Nelson, and Brian C.-S. Liu 10. Immunoprofiling Using NAPPA Protein Microarrays Sahar Sibani and Joshua LaBaer Part III: Protein Function Microarrays 11. High-Throughput Mammalian Two-Hybrid Screening for Protein-Protein Interactions Using Transfected Cell Arrays (CAPPIA) Andrea Fiebitz and Dominique Vanhecke 12. Protein-Protein Interactions: An Application of Tus-Ter Mediated Protein Microarray System Kalavathy Sitaraman and Deb K. Chatterjee 13. Kinase Substrate Interactions Michael G. Smith, Jason Ptacek, and Michael Snyder 14. A Functional Protein Microarray Approach to Characterizing Posttranslational Modifications on Lysine Residues Jun Seop Jeong, Hee-Sool Rho, and Heng Zhu Part IV: Strategies for Validation of Candidate Targets 15. Multiplexed Detection of Antibodies Using Programmable Bead Arrays Karen S. Anderson 16. A Co-Precipitation-Based Validation Methodology for Interactions Identified Using Protein Microarrays Ovidiu Marina, Jonathan S. Duke-Cohan, and Catherine J. Wu Part V: Generation of Proteomic Libraries 17. Development of Expression-Ready Constructs for Generation of Proteomic Libraries Charles Yu, Kenneth H. Wan, Ann S. Hammonds, Mark Stapleton, Joseph W. Carlson, and Susan E. Celniker Part VI: Detection Methods 18. Reverse Phase Protein Microarrays: Fluorometric and Colorimetric Detection Rosa I. Gallagher, Alessandra Silvestri, Emanuel F. Petricoin III, Lance A. Liotta, and Virginia Espina 19. Förster Resonance Energy Transfer Methods for Quantification of Protein-Protein Interactions on Microarrays Michael Schäferling and Stefan Nagl 20. Label-Free Detection with Surface Plasmon Resonance Imaging Christopher Lausted, Zhiyuan Hu, and Leroy Hood Part VII: Data Analysis Techniques for Protein Function Microarrays 21. Data Processing and Analysis for Protein Microarrays David S. DeLuca, Ovidiu Marina, Surajit Ray, Guang L. Zhang, Catherine J. Wu, and Vladimir Brusic 22. Database Resources for Proteomics-Based Analysis of Cancer Guang Lan Zhang, David S. DeLuca, and Vladimir Brusic


Titel: Protein Microarray for Disease Analysis
Untertitel: Methods and Protocols
EAN: 9781493961740
ISBN: 1493961748
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 388
Gewicht: 728g
Größe: H254mm x B178mm x T20mm
Jahr: 2016
Untertitel: Englisch
Auflage: Softcover reprint of the original 1st ed. 2011

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