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Protein Chromatography

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  • 436 Seiten
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This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed ch... Weiterlesen
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This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains keynotes and implementation advice from the experts

Part I Overview Chapters1. A Synopsis of Protein and their PurificationDermot Walls, Gary Cooney, and Sinéad T. Loughran2. Gel-Filtration ChromatographyCiarán Ó'Fágáin, Philip M. Cummins, and Brendan F. O'Connor3. Immunoaffinity Chromatography: Concepts and ApplicationsJenny Fitzgerald, Paul Leonard, Elaine Darcy, Shikha Sharma, and Richard O'Kennedy4. Avoiding Proteolysis During Protein PurificationBarry J. Ryan and Gary T. Henehan5. Scale-Up of Protein Purification: Downstream Processing IssuesJohn Joseph Milne6. Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune SourcesWilliam J.J. Finlay, Laird Bloom, Joanne Grant, Edward Franklin, Deirdre Ní Shúilleabháin, and Orla Cunningham7. Protein Stability: Enhancement and Measurement Ciarán Ó'Fágáin8. Tagging Recombinant Proteins to Enhance Solubility and Aid Purification Sinéad T. Loughran and Dermot WallsPart II Protocols Chapters9. Storage and Lyophilisation of Pure ProteinsCiarán Ó'Fágáin and Keith Colliton<10. Differential Precipitation and Solubilization of ProteinsBarry J. Ryan and Gemma K. Kinsella11. Ion-Exchange Chromatography: Basic Principles and Application Philip M. Cummins, Keith D. Rochfort, and Brendan F. O'Connor12. Protein Quantitation and Analysis of Purity Eva M. Campion, Sinéad T. Loughran, and Dermot Walls13. Purification of Proteins Fused to Maltose-Binding Protein Mario Lebendiker and Tsafi Danieli14. Purification of Poly-Histidine-Tagged ProteinsSinéad T. Loughran, Ronan T. Bree, and Dermot Walls15. Purification of Antibodies Using Affinity ChromatographyElaine Darcy, Paul Leonard, Jenny Fitzgerald, Martin Danaher, Hui Ma, and Richard O' Kennedy16. Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens William J.J. Finlay, Laird Bloom, Sreeja Varghese, Bénédicte Autin, and Orla Cunningham17. Measuring Protein-Protein Interactions Using Biacore Paul Leonard, Stephen Hearty, Hui Ma, and Richard O'Kennedy18. Hydrophobic Interaction Chromatography Brendan F. O'Connor and Philip M. Cummins19. Fast Protein Liquid Chromatography Ashkan Madadlou, Siobhan O'Sullivan, and David Sheehan20. Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems Michael Henry and Paula Meleady21. Strategies for the Purification of Membrane Proteins Sinéad M. Smith22. Antimicrobial Peptide Production and Purification Srinivas Suda, Des Field, and Niall Barron 23. Lectin Affiity Chromatography (LAC)< ` Brendan F. O'Connor, Donal Monaghan, and Jonathan Cawley


Titel: Protein Chromatography
Untertitel: Methods and Protocols
EAN: 9781493964109
ISBN: 1493964100
Format: Fester Einband
Herausgeber: Springer New York
Anzahl Seiten: 436
Gewicht: 1012g
Größe: H260mm x B183mm x T29mm
Jahr: 2016
Untertitel: Englisch
Auflage: 2nd ed. 2017

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