Prostate Cancer

This volume presents methods and protocols on modern analysis of steroid receptor and prostate cancer function. Chapters detail androgen, androgen receptor action, anti-androgen enzalutamide, in vitro and in vivo models, imaging, gene fusions, and help facilitate studies with novel drugs including anti-androgens and chemotherapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Prostate Cancer: Methods and Protocols aims to provide easily reproducible techniques.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Generation of Prostate Cancer Patient-derived Xenografts to Investigate Mechanisms of Novel Treatments and Treatment Resistance.- Methods to Study Angiogenesis in a Mouse Model of Prostate Cancer.- Methodologies Applied to Establish Cell Cultures in Prostate Cancer.- Protocols for Migration and Invasion Studies in Prostate Cancer.- Transplantable Animal Studies and Whole-body Optical Imaging in Prostate Carcinoma.- Protocols for Tissue Microarrays in Prostate Cancer Studies.- Functional Studies on Steroid Receptors.- Protocols for Studies on TMPRSS/ERG in Prostate Cancer.- Protocols for the Study of Taxanes Chemosensitivity in Prostate Cancer.- A Method for Prostate and Breast Cancer Cell Spheroid Cultures using Gelatin Methacryloyl-based Hydrogels.- Protocols for Studies on Genetically Engineered Mouse Models in Prostate Cancer.- Protocols for Studies on Stromal Cells in Prostate Cancer.- Techniques for Evaluation of AR Transcriptional Output and Recruitment to DNA.- NMR-based Prostate Cancer Metabolomics.- Studies on Steroid Receptor Coactivators in Prostate Cancer.