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Poly(ADP-ribose) Polymerase

  • Kartonierter Einband
  • 540 Seiten
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This Methods in Molecular Biology(TM) book shows how PARP proteins act in normal and pathogenic development of organisms, advances... Weiterlesen
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This Methods in Molecular Biology(TM) book shows how PARP proteins act in normal and pathogenic development of organisms, advances knowledge of developmental pathways regulation, and furthers development of new drugs and methods to target PARP-dependent processes.

Poly (ADP-ribose) Polymerases (PARPs) are abundant and ubiquitous proteins that regulate crucial processes of the cell cycle, DNA repair, genomic stability, and transcriptional regulation. Being involved in basic cell functions, PARPs mediate rapid responses to such environmental factors as stress, infection, nutrition and hormonal signals. Whereas PARP inhibitors can suppress tumor growth and proliferation in certain breast, ovarian, and prostate cancers, understanding how PARP controls cellular functions is essential for the development of novel cancer treatments strategies. Divided into three convenient sections, Poly(ADP-Ribose) Polymerase: Methods and Protocols aims to explain how PARP proteins act within the normal development of an organism as well as in pathogenic conditions, seeks to advance the knowledge of developmental pathways regulation, and endeavors to facilitate the development of new therapeutic drugs and methods to target PARP-dependent processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Poly(ADP-Ribose) Polymerase: Methods and Protocols serves as an ideal guide to scientists who wish to continue exploring this exciting and progressive research field.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras


Part I. Biochemical Approaches, In vitro and In vivo

1. Approaches to Detect PARP-1 Activation In vivo, In situ, and In vitro

Girsih M. Shah, Febitha Kandan-Kulangara, Alicia Montoni, Rashmi G. Shah, Julie Brind'Amour, Momchil D. Vodenicharov, and El Bachir Affar

2. Quantification of Poly(ADP-ribose) In vitro: Determination of the (ADP-ribose) Chain Length and Branching Pattern

Rafael Alvarez-Gonzalez and Myron K. Jacobson

3. Assay for Protein Modification by Poly-ADP-Ribose In vitro

Opeyemi A. Olabisi and Chi-Wing Chow

4. Identification of Distinct Amino Acids as ADP-ribose Acceptor Sites by Mass Spectrometry

Florian Rosenthal, Simon Messner, Bernd Roschitzki, Peter Gehrig, Paolo Nanni, and Micahel O. Hottiger

5. Non-covalent Protein Interaction with Poly(ADP-ribose)

Maria Malanga and Felix R. Althaus

6. Non-covalent pADPr Interaction with Protein and Competition with RNA for Binding to Proteins

Yingbiao Ji

7. Affinity-based Assays for the Identification and Quantitative Evaluation of Non-covalent Poly(ADP-ribose)-binding Proteins

Jean-Phillipe Gagné, Jean-François Haince, Émilie Pic, and Guy G. Poirier

8. Detecting and Quantifying pADPr In vivo

Yi-Chen Lai, Rajesh K. Aneja, Margaret A. Satchell, and Robert S. B. Clark

9. Purification of Recombinant Poly(ADP-ribose) Polymerases

Jean-Christophe Amé, Thomas Kalish, Françoise Dantzer, and Valérie Schreiber

10. Methods for Purification of Proteins Associated with Cellular poly(ADP-risbose) and PARP-specific Poly(ADP-ribose)

Jennifer E. Rood, Anthony K. L. Leung, and Paul Chang

11. Purification and Characterization of Poly(ADP-ribosyl)ated DNA Replication/Repair Complexes

Cynthia M. Simbulan-Rosenthal, Dean S. Rosenthal, and Mark E. Smulson

12. PARP1 Genomics: Chromatin Immuno-precipitation Approach using Anti-PARP1 Antibody (ChIP and ChIP-seq)

Niraj Lodhi and Alexei V. Tulin

13. Purification of Human PARP-1 and PARP-1 Domains from E. coli for Structural and Biochemical Analysis

Marie-France Langelier, Jamie L. Planck, Kristin M. Servent, and John M. Pascal

14. Regulation of Chromatin Structure by PARP-1

MiYoung Kim

Part II. Genetic Manipulation Approaches

15. The Use of PARP Inhibitors in Cancer Therapy: Use as Adjuvant with Chemotherapy or Radiotherapy, Use as a Single Agent in Susceptible Patients; Techniques used to Identify Susceptible Agents

Sydney Shall, Terry Gaymes, Farzin Farzaneh, Nicola Curtin, and Ghulam J. Mufti

16. Quantification of PARP Activity in Human Tissues: Ex vivo Assays in Blood Cells, and Immunohistochemistry in Human Biopsies

Eszter M. Horvath, Zsuzsanna K. Zsengellér, and Csaba Szabo

17. Assay for Hypermethilation of the BRCA1 Gene Promoter in Tumor Cells to Predict Sensitivity to PARP-Inhibitor Therapy

Ilsiya Ibragimova and Pauls Cairns

18. Generation of Poly(ADP-ribosyl)ation Deficient Mutants of the Transcription Factor, CTCF

Dawn Farrar, Igor Chernukhin, and Elena Klenova

19. Phenotypic Characterization of Parp-1 and Parp-2 Deficient Mice and Cells

Christian Boehler, Laurent Gauthier, Jose Yelamos, Aurélia Noll, Valérie Schreiber, and Françoise Dantzer

20. Poly(ADP-ribosyl)ation Pathways in Mammals: The Advantage of Murine PARG Null Mutation

Yiran Zhou and David W. Koh

21. Genetic Approaches to Targeting Multiple PARP Genes in a Mammalian Genome

Mirella L. Meyer-Ficca and Ralph G. Meyer

22. Approaches to Separate and Identify Poly(ADP-ribosyl)ated Proteins using PARG Knockout Drosophila

Hiroto Nodono, Akiko Hamada, Yasuhito Kuroda, Kazuo Kamemura, Makoto Hasegawa, Toru Komiya, Machiko Kondo, Fan-Sik Che, Shuji Hanai, and Masanao Miwa

23. Immuno-localization of poly-ADP-Ribose on Drosophila Polytene Chromosomes

Anna Sala and Davide F. V. Corona

24. The Immuno-detection of pADPr in Drosophila Tissues: Immunostaining of Thick Sections; Immuno-EM

Michael Jarnik

25. Poly(ADP-ribose) Metabolism Analysis in the Nematode Caenorhabditis elegans

Jean-François St-Laurent and Serge Desnoyers

26. Genetic Dissection of PARylation in the Filamentous Fungus Neurospora crassa

Gregory O. Kothe

27. Purification of the Poly-ADP-ribose Polymerase like Thermozyme from the Archaeon Sulfolobus Solfataricus

Anna De Maio, Elena Porzio, Ida Romano, Barbara Nicolaus, and Maria Rosaria Faraone Mennella

28. Strategies Employed for the Development of PARP Inhibitors

Stacie Canan, Karen Maegley, and Nicola Curtin

29. Small-molecule Collection and High-throughput Colorimetic Assay to Identify PARP-1 Inhibitors

Elena Kotova, Aaron D. Pinnola, and Alexei V. Tulin


Titel: Poly(ADP-ribose) Polymerase
Untertitel: Methods and Protocols
EAN: 9781493962150
ISBN: 1493962159
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 540
Gewicht: 1003g
Größe: H254mm x B178mm x T28mm
Jahr: 2016
Untertitel: Englisch
Auflage: Softcover reprint of the original 1st ed. 2011

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