Beschreibung

Recent years have seen significant advancements in the development of enabling technologies that facilitate the study of Transcription Factors (TFs). TFs are pivotal in the regulation of plant development, reproduction, intercellular signaling, response to environment, cell cycle, and metabolism. Plant Transcription Factors: Methods and Protocols offers a comprehensive approach by covering the basic concepts as well as the detailed protocols of a series of commonly used tools for investigating plant TFs. From discussing select TF families in plants to presenting approaches for identifying them, methods are covered to verify the function, to identify protein interactions in which TFs are involved, and how the interactions are mediated. Increasing examples of TFs that function non-cell-autonomously are being discovered and methods to assess intercellular trafficking are also addressed. A section is devoted to examining interaction with DNA, and the volume concludes with a discussion of directed evolution to generate transcription factors that can more efficiently control desired processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Plant Transcription Factors: Methods and Protocols serves as an ideal guide to seasoned plant molecular biologists as well as scientists new to the field of TFs and provides many necessary methods to all scientists who are interested in exploring the functions of transcription factors.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras

Inhalt
Section I. Introduction1. MADS & More: Transcription Factors that Shape the Plant Rainer Melzer and Günter TheißenSection II. Identification of Transcription Factors2. In Silico Mining and PCR Based Approaches to Transcription Factor Discovery in Non-Model Plants: Gene Discovery of the WRKY Transcription Factors in Conifers Jun-Jun Liu and Yu Xiang3. Isolation of Plant Transcription Factors Using a Yeast One-Hybrid System Tatiana Pyvovarenko and Sergiy Lopato4. A Transposon Based Activation Tagging System for Gene Function Discovery in Arabidopsis Nayelli Marsch-MartínezSection III. Functional Verification5. CRES-T, An Effective Gene Silencing System Utilizing Chimeric Repressors Nobutaka Mitsuda, Kyoko Matsui, Miho Ikeda, Masaru Nakata, Yoshimi Ooshima, Yukari Nagatoshi, and Masaru Ohme-Takagi6. Analysis of a Transcription Factor Using Transient Assay in Arabidopsis Protoplasts Yuji Iwata, Mi-Hyun Lee, and Nozomu Koizumi7. Microarray-Based Identification of Transcription Factor Target Genes Maartje Gorte, Anneke Horstman, Robert B. Page, Renze Heidstra, Arnold Stromberg, and Kim BoutilierSection IV. Protein-Protein Interaction and Other Means for Activity Control8. Yeast Protein-Protein Interaction Assays and Screens Stefan de Folter and Richard G.H. Immink 9. Mapping Functional Domains of transcription factors Ling Zhu and Enamul Huq10. Bimolecular fluorescence complementation as a tool to study interactions of regulatory proteins in plant protoplasts Sitakanta Pattanaik, Joshua R. Werkman, and Ling Yuan11. Isolation of transcription factor complexes from Arabidopsis cell suspension cultures by tandem affinity purification Jelle Van Leene, Dominique Eeckhout, Geert Persiau, Eveline Van De Slijke, Jan Geerinck, Gert Van Isterdael, Erwin Witters, and Geert De Jaeger12. Assaying Transcription Factor Stability Jasmina Kurepa and Jan A. Smalle13. How to assess the intercellular trafficking of transcription factors Munawar Ahmad, Won Kyong Cho, Yeonggil Rim, Lijun Huang, and Jae-Yean KimSection V. Protein-DNA Interaction14. SELEX (Systematic Evolution of Ligands by EXponential Enrichment), as a powerful tool for deciphering the protein-DNA interaction space Chenglin Chai, Zidian Xie, and Erich Grotewold15. Footprinting and Missing Nucleoside Analysis of Transcription Factor-DNA Complexes Ivana L. Viola and Daniel H. Gonzalez16. Chromatin immunoprecipitation to verify or to identify In Vivo protein-DNA interactions Yumei Zheng and Sharyn E. Perry17. Visualizing and characterizing In Vivo DNA-Binding Events and Direct Target Genes of Plant Transcription Factors Jose M. Muiño, Gerco C. Angenent, and Kerstin Kaufmann18. Mapping In Vivo Protein-DNA Interactions in Plants by DamID, a DNA Adenine Methylation-Based Method Sophie Germann and Valérie GaudinSection VI. Engineering Metablolic Pathways using Transcription Factors19. Directed Evolution through DNA Shuffling for the Improvement and Understanding of Genes and Promoters Joshua R. Werkman, Sitakanta Pattanaik, and Ling Yuan

Produktinformationen

Titel:	Plant Transcription Factors
Untertitel:	Methods and Protocols
Editor:	Sharyn E. Perry   Ling Yuan
EAN:	9781617791536
ISBN:	1617791539
Format:	Fester Einband
Herausgeber:	Humana Press
Anzahl Seiten:	360
Gewicht:	874g
Größe:	H260mm x B183mm x T24mm
Jahr:	2011
Untertitel:	Englisch
Auflage:	2011