Plant Systems Biology

In this authoritative guide, expert investigators provide cutting-edge chapters dealing with modern plant systems biology approaches. This work provides the kind of detailed description and implementation advice that is crucial for getting optimal results.

As a fairly new and expansive area of study, plant systems biology has been approached by scientists from fields as varied as plant physiology and astrophysics, creating a wide variety of techniques and methods to further this vital research. In Plant Systems Biology, expert investigators provide cutting-edge chapters dealing with diverse subjects such as systems biology of plant gene expression to analysis of networks, pathways, specific statistical issues and novel computational tools, imaging-based tools as well as chemical genetic, metabolomic and integrative methods that cannot be easily pigeonholed. As a volume in the highly successful Methods in Molecular Biology ™ series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results.

Authoritative and comprehensive, Plant Systems Biology is an ideal guide for all researchers who wish to contribute to the evolution of the vibrant field of plant biology.

Authoritative guide to modern plant systems biology approaches Up-to-date compilation, written by leading experts in the field Presents a combination of high throughput and boutique, one-of-a-kind methodologies Covers both experimental and computational/statistical issues in plant systems biology

Inhalt
I: Systems Biology of Plant Gene Expression.- Gene-Specific and Genome-Wide ChIP Approaches to Study Plant Transcriptional Networks.- Genome-Wide Analysis of RNA-Protein Interactions in Plants.- Whole-Genome Microarrays: Applications and Technical Issues.- Manipulating Large-Scale Arabidopsis Microarray Expression Data: Identifying Dominant Expression Patterns and Biological Process Enrichment.- Applications of Ultra-high-Throughput Sequencing.- Isolation of Plant Polysomal mRNA by Differential Centrifugation and Ribosome Immunopurification Methods.- Chromatin Charting: Global Mapping of Epigenetic Effects.- Clone-Based Functional Genomics.- II: Networks, Pathways, Statistical Issues, and Novel Computational Tools.- Challenges and Approaches to Statistical Design and Inference in High-Dimensional Investigations.- Discrete Dynamic Modeling with Asynchronous Update, or How to Model Complex Systems in the Absence of Quantitative Information.- Quantification of Variation in Expression Networks.- Co-expression Analysis of Metabolic Pathways in Plants.- Integration of Metabolic Reactions and Gene Regulation.- Applying Word-Based Algorithms: The IMEter.- III: Imaging.- Live-Imaging and Image Processing of Shoot Apical Meristems of Arabidopsis thaliana.- Computer Vision as a Tool to Study Plant Development.- IV: Chemical Genetic, Metabolomic, and Integrative Methods for Plant Systems Biology.- Metabolomics of Plant Volatiles.- Chemical Genomics Approaches in Plant Biology.- Comparison of Quantitative Metabolite Imaging Tools and Carbon-13 Techniques for Fluxomics.- Democratization and Integration of Genomic Profiling Tools.