Plant Signal Transduction

Recent research has greatly expanded our understanding of the sophisticated molecular network of responses in plants. Here, an international panel of experts provides well-established methods vital to analyzing plant signal transduction on the molecular level.

During recent years, research has greatly expanded our understanding of the sophisticated molecular network of responses which enable plants to develop, survive and propagate under a wide range of conditions. In Plant Signal Transduction: Methods and Protocols, an international panel of experts provide well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in planta analyses and the proteins involved in signal transduction in order to aid with the establishment of laboratory techniques or the modification of the protocols for other plants. As part of the highly successful Methods in Molecular Biology™ series, the chapters include brief introductions to the subject, lists of necessary materials, readily reproducible laboratory protocols, and tips on trouble-shooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Plant Signal Transduction: Methods and Protocols will benefit plant scientists wishing to improve their experimental approaches and delve further into this exciting and important field of study

Offers step-by-step, established and reproducible laboratory protocols written by experts in the field of plant signal transduction and molecular physiology Focuses on novel and emerging techniques to analyze signaling mutants, post-translational protein modification, protein-protein and protein-nucleic acid interactions Covers a broad spectrum of signal transduction protocols, from a general introduction to signalling in plants to technical applications for investigating specific problems Combines basic bench techniques, sophisticated microscopical and mass spectrometrical applications, molecular strategies for investigating interaction partners and guides for bioinformatics promoter analyses

Inhalt
Multiple Dimensions in Plant Signal Transduction: An Overview.- Phenotyping of Arabidopsis Mutants for Developmental Effects of Gene Deletions.- Phenotyping of Abiotic Responses and Hormone Treatments in Arabidopsis.- Accurate Real-time Reverse Transcription Quantitative PCR.- Probing Spatio-temporal Intracellular Calcium Variations in Plants.- Dynamic Redox Measurements with Redox-Sensitive GFP in Plants by Confocal Laser Scanning Microscopy.- Imaging of Reactive Oxygen Species In vivo.- Identification of Thioredoxin Targeted Proteins Using Thioredoxin Single-Cysteine Mutant-Immobilized Resin.- Determination of in vivo Protein Phosphorylation in Photosynthetic Membranes.- Examining Protein Stability and Its Relevance for Plant Growth and Development.- Probing Circadian Rhythms in Chlamydomonas reinhardtii by Functional Proteomics.- Bimolecular Fluorescence Complementation (BiFC) to Study Protein-protein Interactions in Living Plant Cells.- Fluorescence Cross-Correlation Spectroscopy of Plant Proteins.- The Determination of Protein-protein Interactions by the Mating-based Split-ubiquitin system (mbSUS).- Functional Complementation of Yeast Mutants to Study Plant Signalling Pathways.- Phosphatase Activities Analyzed by in vivo Expressions.- Chromatin Immunoprecipitation Experiments to Investigate In Vivo Binding of Arabidopsis Transcription Factors to Target Sequences.- Fluorescence-based Electrophoretic Mobility Shift Assay in the Analysis of DNA-binding Proteins.- Analysis of Plant Regulatory DNA sequences by the Yeast-One-Hybrid Assay.- Analysis of Plant Regulatory DNA Sequences by Transient Protoplast Assays and Computer Aided Sequence Evaluation.- RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System.