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Plant Receptor Kinases

  • Fester Einband
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This is volume presents protocols relevant for plant receptor kinases(PRK) both in development and defence. Chapters guide readers... Weiterlesen
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Beschreibung

This is volume presents protocols relevant for plant receptor kinases(PRK) both in development and defence. Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways. Written in the highly successful Methods

in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Receptor Kinases: Methods and Protocols aims to ensure successful results in the further study of this vital field.


Inhalt

PART I Expression and Purification of PRKs

1. Expression of Plant Receptor Kinases in E. coli

Moneeza Akbar Agha, David Lightfoot, and Ahmed Jawaad Afzal

2. Expression of Plant Receptor Kinases in Yeast

María Laura Barberini and Jorge Muschietti

3. Expression of Plant Receptor Kinases in Tobacco BY-2 Cells

Hidefumi Shinohara and Yoshikatsu Matsubayashi

4. Cell-free Synthesis of Plant Receptor Kinases

Akira Nozawa, Keiichirou Nemoto, Shunsuke Nomura, Satoshi Yamanaka, Kohki Kido, and Tatsuya Sawasaki

5. Purification of Plant Receptor Kinases from Plant Plasma Membranes

Jin Suk Lee

PART II Ligand Binding and Activation of PRKs

6. Photoaffinity Labeling of Plant Receptor Kinases

Hidefumi Shinohara and Yoshikatsu Matsubayashi

7. Quantitative Detection of Oxidative Burst upon Activation of Plant Receptor Kinases

Markus Albert and Ursula Fürst

PART III In silico Methods for Identification and Analyses of PRKs

8. Extraction and Curation of Gene Models for Plant Receptor Kinases for Phylogenetic Analysis

Aleksia Vaattovaara, Jarkko Salojärvi, and Michael Wrzaczek

9. In silico Prediction of Ligand Binding Sites of Plant Receptor Kinases using Conservation Mapping

Russell J. S. Orr and Reidunn B. Aalen

PART IV Identification of Enzymatic Functions and Interacting Partners of PRKs

10. Co-immunoprecipitation of Plant Receptor Kinases

María Laura Barberini and Jorge Muschietti

11. Autophosphorylation Assays using Plant Receptor Kinases Synthesized in Cell-free Systems

Keiichirou Nemoto, Akira Nozawa, Satoshi Yamanaka, Shunsuke Nomura, Kohki Kido, and Tatsuya Sawasaki

12. Investigation of Autophosphorylation Sites of Plant Receptor Kinases and Phosphorylation of Interacting Partners

Matthew R Meyer, Shweta Shah, and A. Gururaj Rao

13. In vitro Assessment of Guanylyl Cyclase Activity of Plant Receptor Kinases

Misjudeen Raji and Chris Gehring

14. BiFC Assay to Detect Calmodulin Binding to Plant Receptor Kinases

Cornelia Fischer, Margret Sauter, and Petra Dietrich

15. Pull Down Assay to Characterize Ca2+/Calmodulin Binding to Plant Receptor Kinases

Christine Kaufmann and Margret Sauter

PART V Imaging of PRK Dynamics

16. The use of FRET/FLIM to Study Proteins Interacting with Plant Receptor Kinases

Stefanie Weidtkamp-Peters and Yvonne Stahl

17. Detection and Analyses of Endocytosis of Plant Receptor Kinases

Jenna Loiseau and Silke Robatzek

Produktinformationen

Titel: Plant Receptor Kinases
Untertitel: Methods and Protocols
Editor:
EAN: 9781493970629
ISBN: 1493970623
Format: Fester Einband
Herausgeber: Springer New York
Anzahl Seiten: 208
Gewicht: 598g
Größe: H260mm x B183mm x T17mm
Jahr: 2017
Auflage: 1st ed. 2017
Land: GB

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