Plant Hormones

Given the rapid increase in our understanding of plant hormone biology, this second edition of a comprehensive review could not have come at a better time. In its chapters, expert researchers explore the latest approaches to understanding plant hormone action.

The last 10 years have witnessed an explosion in our understanding of plant h- mones. The often vague models of hormone action developedover decadeshave been replaced in short order by detailed molecular models that include receptors and in many cases downstream signal transduction components. Given the rapid progress in understanding the mechanism of action of plant growth regulators, a technical review of hormone methodology is timely. Our book focuses on genetic, biochemical, ana- tical and chemical biological approaches for understanding and dissecting plant h- mone action. The greatest strides in plant hormone biology have come, by and large, from the use of genetic methods to identify receptors and we dedicate a chapter to general genetic methods of analysis using the model system Arabidopsis thaliana. A cluster of chapters focuses on biochemical methods for documenting interactions betweenhormonesand their receptors. Theimportance of these assays is tremendous; receptorligand interactions in animal model systems have been the cornerstones of pharmacological and medicinal chemical assays that have enabled identification of selective and non-selective agonists and antagonists that can be used to further probe and dissect questions of receptor function. This is likely to be a major new frontier in plant hormone research.

Cutting edge approaches for studying plant hormones and their receptors that have been perfected over the last 5 year Provides the basics of the LC-MS and GC-MS methods that are critical to modern hormone research Includes advanced protocols for mutant identification and assaying hormone responses in heterologous systems such as yeast Offers a concise overview of current methods and reagents needed to dissect plant signalling pathways using chemical genetic methods Features robust protocols for the use of high-throughput sequencing technologies for microRNA analysis Includes supplementary material: sn.pub/extras

Klappentext

The last ten years have witnessed a monumental increase in our understanding of plant hormones. A decade ago, scientists were forced to rely on vague ideas about hormone action; today, these have been replaced by detailed molecular models. Given the rapid increase in our understanding of plant hormone biology, this comprehensive review could not have come at a better time. In Plant Hormones: Methods and Protocols, Second Edition, expert researchers explore the most current genetic, biochemical, analytical and chemical biological approaches for understanding plant hormone action, providing a concise overview of methods and reagents needed to dissect plant signalling pathways using chemical genetic methods. Chapters address such topics as genetic methods of analysis using the model system Arabidopsis thaliana, biochemical methods for documenting interactions between hormones and receptors, the use of high-throughput sequencing technologies for microRNA analysis, and analytical approaches for measuring endogenous plant hormone levels, including LC-MS and GC-MS based methods. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Innovative and cutting edge, Plant Hormones: Methods and Protocols, Second Edition is an essential guide for all plant scientists who are facing the complex and challenging questions that will determine the future of this vital field.

Inhalt
Using Reverse Genetics to Develop Small Knockout Collections for Specific Biological Questions.- Visualizing Auxin Transport Routes in Arabidopsis Leaf Primordia.- Gene Expression Analyses for Elucidating Mechanisms of Hormonal Action in Plants.- Measurement of Plant Hormones by Liquid ChromatographyMass Spectrometry.- Measurement of Abscisic Acid and Gibberellins by Gas Chromatography/Mass Spectrometry.- Pull-Down Assays for Plant Hormone Research.- Binding Assays for Brassinosteroid Receptors.- Binding Assays for Abscisic Acid Receptors.- Cytokinin Sensing Systems Using Microorganisms.- Hormone-Binding Assay Using Living Bacteria Expressing Eukaryotic Receptors.- Discovery of Plant MicroRNAs and Short-Interfering RNAs by Deep Parallel Sequencing.- The Use of Chemical Genomics to Investigate Pathways Intersecting Auxin-Dependent Responses and Endomembrane Trafficking in Arabidopsis Thaliana.