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Plant Epigenetics

  • Fester Einband
  • 264 Seiten
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Leseprobe
This volume provides a variety of protocols to analyze various epigenetic changes, including differential expression of non-coding... Weiterlesen
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Beschreibung

This volume provides a variety of protocols to analyze various epigenetic changes, including differential expression of non-coding RNAs, changes in DNA methylation, and histone modifications in plants. Chapters detail protocols with different degrees of complexity, and describe bioinformatics approaches for data processing and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Epigenetics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.




Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts



Klappentext

This
volume provides a variety of protocols to analyze various epigenetic
changes, including differential expression of non-coding RNAs, changes in DNA
methylation, and histone modifications in plants. Chapters detail protocols
with different degrees of complexity, and describe bioinformatics approaches
for data processing and analysis. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.


Authoritative and
cutting-edge, Plant Epigenetics: Methods and Protocols, Second Edition
aims to ensure successful results in the further study of this vital field.





Zusammenfassung

This volume provides a variety of protocols to analyze various epigenetic changes, including differential expression of non-coding RNAs, changes in DNA methylation, and histone modifications in plants. Chapters detail protocols with different degrees of complexity, and describe bioinformatics approaches for data processing and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Epigenetics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.


 



Inhalt
1. Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA Binding Analyses in ArabidopsisWanhui You, Stéphane Pien, and Ueli Grossniklaus 2. Chromatin Conformation Capture-Based Analysis of Nuclear ArchitectureStefan Grob and Ueli Grossniklaus 3. Metaanalysis of Genome-Wide Chromatin DataJulia Engelhorn and Franziska Turck 4. Localization of Mirnas by In Situ Hybridization in Plants using Conventional Oligonucleotide ProbesSara Hernández-Castellano, Geovanny I. Nic-Can, Clelia De-La-Peña 5. COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis of Locus-Specific Changes in Methylation Patterns Andriy Bilichak and Igor Kovalchuk 6. Analysis of Global Genome Methylation Using the Cytosine-Extension AssayAndriy Bilichak and Igor Kovalchuk 7. In Situ Analysis Of DNA Methylation in PlantsPalak Kathiria and Igor Kovalchuk < 8. Analysis of DNA Hydroxymethylation in Arabidopsis Using Colorimetric Assay Andrey Golubov and Igor Kovalchuk 9. Analysis of DNA Cytosine Methylation Patterns using Methylation Specific Amplification Polymorphism (MSAP)María Ángeles Guevara, Nuria De María, Enrique Sáez-Laguna, María Dolores Vélez, María Teresa Cervera, and José Antonio Cabezas 10. Differentially Methylated Region - Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify Dmrs in Uncharacterized GenomesPavlina Sasheva Ueli Grossniklaus 11. Analysis of Small RNA Populations Using Hybridization to DNA Tiling Arrays Martine Boccara, Alexis Sarazin, Bernard Billoud, Agnes Bulski, Louise Chapell, David Baulcombe, and Vincent Colot 12. Northern Blotting Techniques for Small RNAsTodd Blevins 13. Stem-Loop QRT-PCR for the Detection of Plant MicrornasErika Varkonyi-Gasic 14. Profiling New Small RNA SequencesMasayuki Tsuzuki and Yuichiro Watanabe 15. Small RNA Library Preparation and Illumina Sequencing in PlantsAndriy Bilichak, Andrey Golubov, and Igor Kovalchuk 16. Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow

Produktinformationen

Titel: Plant Epigenetics
Untertitel: Methods and Protocols
Editor:
EAN: 9781489977069
ISBN: 1489977066
Format: Fester Einband
Herausgeber: Springer US
Anzahl Seiten: 264
Gewicht: 700g
Größe: H260mm x B183mm x T20mm
Jahr: 2016
Untertitel: Englisch
Auflage: 2nd ed. 2017

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