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Plant Cell Division

  • Kartonierter Einband
  • 243 Seiten
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This volume aims to present a large panel of techniques for the study of Plant Cell Division. Plant Cell Division: Methods and Pro... Weiterlesen
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This volume aims to present a large panel of techniques for the study of Plant Cell Division. Plant Cell Division: Methods and Protocols captures basic experimental protocols that are commonly used to study plant cell division processes, as well as more innovative procedures. Chapters are split into five parts covering several different aspect of plant cell division such as, cell cultures for cell division studies, cell cycle progression and mitosis, imaging plant cell division, cell division and morphogenesis, and cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Cell Division: Methods and Protocols is a valuable tool for the study of plant cell division at both the cellular and molecular levels, and in the context of plant development.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Part I: Cell Cultures for Cell Division Studies1. Synchronization Of Green Algae By Light And Dark Regimes For Cell Cycle and Cell Division Studies Monika Hlavová, Milada Vítová, and Kateina Biová 2. Plant Cell Division Analyzed by Transient Agrobacterium-Mediated Transformation of Tobacco BY-2 Cells Henrik Buschmann Part II: Cell Cycle and Mitosis3. Identifying Gene Regulatory Networks In Arabidopsis By In Silico Prediction, Yeast-1-Hybrid and Inducible Gene Profiling Assays Erin E. Sparks and Philip N. Benfey4. Cytrap Marker Systems for In Vivo Visualization of Cell Cycle Progression in Arabidopsis Shiori Sugamata Aki and Masaaki Umeda 5. Imaging Nuclear Morphology and Organization in Cleared Plant Tissues Treated with Cell Cycle Inhibitors José Dijair Antonino De Souza Junior, Maria Fatima Grossi De Sa, Gilbert Engler and, Janice De Almeida Engler 6. Isolation of Plant Nuclei at Defined Cell Cycle Stages Using Edu Labeling and Flow Cytometry Emily E. Wear, Lorenzo Concia, Ashley M. Brooks, Emily A. Markham, Tae-Jin Lee, George C. Allen, William F. Thompson, and Linda Hanley-Bowdoin 7. Preparation and Fluorescent Analysis of Plant Metaphase Chromosomes Trude Schwarzacher 8. Fluorescence In Situ Hybridization On Rice Chromosomes Yafei Li and Zhukuan Cheng Part III Imaging Plant Cell Division9. Dynamics of the Plant Nuclear Envelope During Cell Division David E Evans and Katja Graumann 10. Immunolabeling Of Nuclei/Chromosomes In Arabidopsis ThalianaMichael Sandmann, Joerg Fuchs, and Inna Lermontova 11. In Vivo Imaging Of Microtubules Organization In Dividing Giant Cell Marie-Cecile Caillaud and Bruno Favery Part IV: Cell Division and Morphogenesis12. Real-Time Lineage Analysis to Study Cell Division Orientation in the Arabidopsis Shoot MeristemCory J. Tobin and Elliot M. Meyerowitz 13. Cell Proliferation Analysis Using Edu Labeling In Whole Plant and Histological Samples Of ArabidopsisAnita Kazda, Svetlana Akimcheva, J. Matthew Watson, and Karel Riha 14. Studying Cell Division Plane Positioning in Early-Stage Embryos Katia Belcram, Jean-Christophe Palauqui, and Martine Pastuglia Part V: Cytokinesis15. Characterization Oof Cytokinetic Mutants using Small Fluorescent Probes Andrei Smertenko, Panagiotis Moschou, Laining Zhang, Deirdre Fahy, and Peter Bozhkov 16. Using Live Cell Markers in Maize to Analyze Cell Division Orientation and Timing Carolyn G. Rasmussen 17. Ratiometric Fluorescence Live Imaging Analysis of Membrane Lipid Order in Arabidopsis Mitotic Cells Using a Lipid Order-Sensitive Probe Patricia Gerbeau-Pissot, Christophe Der, Markus Grebe, and Thomas Stanislas


Titel: Plant Cell Division
Untertitel: Methods and Protocols
EAN: 9781493979882
ISBN: 978-1-4939-7988-2
Format: Kartonierter Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 243
Gewicht: 614g
Größe: H12mm x B253mm x T178mm
Jahr: 2019
Auflage: Softcover reprint of the original 1st ed. 2016

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