Physical Exercise and Natural and Synthetic Products in Health and Disease

This detailed book explores protocols with the aim of testing aerobic exercise, resistance training, special diets, additives and natural products, which have led to new insights into the physiological and molecular aspects of health and disease. Many of these approaches have contributed to significant improvements in disease areas such as cardiovascular disease, cognitive dysfunction, diabetes, frailty, gliobastoma, metabolic syndrome, obesity, oxidative stress, and various cancers. This collection also provides important information on disease mechanisms and novel drug targets as each protocol is presented in the context of specific chronic diseases or different therapeutic areas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and reflective of research from all around the globe, Physical Exercise and Natural and Synthetic Products in Health and Disease serves as an ideal guide for researchers in the areas of chronic disease, exercise, and nutrition, as well as to clinical scientists, physicians, and pharmacologists as it gives insights into possibilities for the development of novel therapeutics and the means of monitoring therapeutic response through the measurement of molecular and physiometric biomarkers.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

The New Frontier of Three-Dimensional Culture Models to Scale Up Cancer Research.- Therapeutic Effects of Resveratrol on Non-Alcoholic Fatty Liver Disease through Inflammatory, Oxidative Stress, Metabolic, and Epigenetic Modifications.- A Matter of Fat: Body Fat Distribution and Cardiometabolic Disease in Africa.- PGK1: An Essential Player in Modulating Tumor Metabolism.- The Association of Reproductive Aging with Cognitive Function in Sub-Saharan African Women.- The Effects of Exercise on Lipid Biomarkers.- Exercise Training Protocols to Improve Obesity, Glucose Homeostasis, and Subclinical Inflammation.- A Protocol of Intradialytic Exercise for Improvements in Inflammatory Status, Body Composition, and Functional Capacity.- Evaluating the Anticancer Activity of Natural Products Using a Novel 3D Culture Model.- Evaluation of Antidiabetic Properties of Adenosma bracteosum Bonati Extracts in Mice with Streptozotocin-Induced Diabetes.- Testing the Effects of Cinnamon Extract Supplementation on Inflammation and Oxidative Stress Induced by Acrylamide.- Proteomic Mapping of the Human Myelin Proteome.- Testing for Thyroid Peroxidase and Anti-Neuronal Antibodies in Depression and Schizophrenia.- Evaluation of Antimicrobial and Anticancer Activities of Bouea macrophylla Ethanol Extract.- Evaluation of Anti-Hepatocellular-Cancer Properties of -Sitosterol and -Sitosterol-Glucoside from Indigofera zollingeriana Miq.- Label-Free Electrochemical Biosensors to Evaluate the Antioxidant Effect of Tocopherol in Ultraviolet Radiation.- qRT-PCR Analysis of GLUT-4 and Assessment of Trolox as an Effective Antioxidant in Diabetic Cardiomyoblasts.- Flow Cytometric Analysis of Hyperglycemia-Induced Cell Death Pathways in Cardiomyoblasts.- Vietnamese Medicinal Plants as Potential Resources to Explore New Anti-Cancer and Anti-Inflammation: Established Assays for Pharmacological Tests.- Testing the Effect of Curcumin on Proliferative Capacity of Colorectal Cancer Cells.- Assessment of Topical and Transdermal Penetration of Curcuma heyneana Rhizome Extract in Rat Skin: Histological Analysis.- Measuring the Effects of Berberine on Serum Pro-OxidantAntioxidant Balance in Metabolic Syndrome.- Testing the Anti-Inflammatory Effects of Curcuminoids in Patients with Colorectal Cancer.- Analytical Methods and Bioassays for Cytotoxicity and Anti-Diabetic Properties of Aquilaria Crassna Leaf Extracts in HepG2 Cells.- Testing the Physical and Molecular Effects of Nutritional Supplements and Resistance Exercise in Middle-Aged Females.- Analysis of Cytotoxic Effects of Zerumbone in Malignant Glioblastoma Cells.- Protocol for Testing the Potential Antioxidant Effects of Curcuminoids on Patients with Type 2 Diabetes Mellitus.- The Detection of Toxic Compounds in Extracts of Callilepis laureola (Ox-Eye Daisy) and Senecio latifolius (Ragwort) by Ultra-Performance Liquid Chromatography Mass Spectrometry (UPLC-MS/MS).- Impact of Curcumin on Hepatic Low-Density Lipoprotein Uptake.