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This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosph... Weiterlesen
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This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins. Phosphoproteomics Methods and Protocols, Second Edition guides readers through different labeling strategies for quantitative phosphoproteomics; high-throughput mass spectrometry-based phosphoproteome analyses and phospho flow cytometry; and bioinformatics strategies for phosphoproteomics data analysis and integration. Additional protocols concentrate on the identification of kinase-substrate relationships by both high- and low-throughput approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Phosphoproteomics Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Part I: Techniques for Tagging Phospho-Moieties 1. Thiol-ene Enabled Detection of Thiophosphorylation as a Labeling Strategy for PhosphoproteinsKaelyn E. Wilke and Erin E. Carlson 2. Phosphopeptide Detection with Biotin-Labeled Phos-tagEmiko Kinoshita-Kikuta, Eiji Kinoshita and Tohru Koike 3. Phosphopeptide Enrichment by Covalent Chromatography after Solid Phase Derivatization of Protein Digests on Reversed Phase Supports Heinz Nika, Ruth Hogue Angeletti, and David H. Hawke Part II: Labeling Techniques for Quantitative Phosphoproteomics 4. Peptide Labeling Using Isobaric Tagging Reagents for Quantitative PhosphoproteomicsLei Cheng, Trairak Pisitkun, Mark A. Knepper, and Jason D. Hoffert 5. Identification of Direct Kinase Substrates Using Analogue-Sensitive AllelesDaniel A. Rothenberg, Elizabeth A. Gordon, Forest M. White, and Sebastian Lourido 6. Quantitative Analysis of Tissue Samples by Combining iTRAQ Isobaric Labeling with Selected/Multiple Reaction Monitoring (SRM/MRM)Ryohei Narumi and Takeshi Tomonaga Part III: Phosphopeptide Enrichment Strategies 7. Enrichment Strategies in PhosphoproteomicsAlexander Leitner 8. Phosphopeptide Enrichment by Immobilized Metal Affinity ChromatographyTine E. Thingholm and Martin R. Larsen 9. The use of Titanium Dioxide for Selective Enrichment of Phosphorylated PeptidesTine E. Thingholm and Martin R. Larsen 10. Sequential Elution from IMAC (SIMAC) An Efficient Method for Enrichment and Separation of Mono- and Multi-Phosphorylated PeptidesTine E. Thingholm and Martin R. Larsen 11. Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and FractionationKasper Engholm-Keller and Martin R. Larsen 12. Offline High pH Reversed-Phase Peptide Fractionation for Deep Phosphoproteome CoverageTanveer S Batth, Jesper V Olsen 13. Phosphopeptide Enrichment Using Various Magnetic Nanocomposites An OverviewÍris L. Batalha and Ana Cecília A. Roque Part IV: Phosphoprotein Analysis by Mass Spectrometry 14. Two Dimensional Gel Electrophoresis-Based Plant Phosphoproteomics Chao Han and Pingfang Yang 15. Variable Digestion Strategies for Phosphoproteomics AnalysisHumberto Gonczarowska-Jorge, Margherita Dell'Aica, Clarissa Dickhut, and René P. Zahedi 16. On-line LC-FAIMS-MS/MS for the Analysis of Phosphorylation in ProteinsHongyan Zhao, Andrew J. Creese, and Helen J. Cooper 17. Simple and Reproducible Sample Preparation for Single Shot Phosphoproteomics With High SensitivityRosa R. Jersie-Christensen, Abida Sultan, and Jesper V. Olsen Part V: High-throughput Studies of the Phosphoproteome 18. Identification of Direct Kinase Substrates via Kinase Assay Linked PhosphoproteomicsLiang Xue, Justine V. Arrington, and W. Andy Tao 19. Phosphoprotein Detection by High-Throughput Flow CytometryJohannes Landskron and Kjetil Taskén Part VI: Bioinformatics Resources for Phosphoproteomics Analyses 20. Resources for Assignment of Phosphorylation Sites on Peptides and Proteins Vaishnavi Ravikumar, Boris Macek, and Ivan Mijakovic 21. From Phosphosites to KinasesStephanie Munk, Jan C. Refsgaard, and Jesper V. Olsen, and Lars J. Jensen 22. Search Databases and Statistics Pitfalls and Best Practices in PhosphoproteomicsJan C. Refsgaard, Stephanie Munk, and Lars J. Jensen 23. Systems Analysis for Interpretation of Phosphoproteomics DataStephanie Munk, Jan C. Refsgaard, and Jesper V. Olsen


Titel: Phospho-Proteomics
Untertitel: Methods and Protocols
EAN: 9781493950010
ISBN: 1493950010
Format: Kartonierter Einband
Herausgeber: Springer New York
Anzahl Seiten: 380
Gewicht: 713g
Größe: H254mm x B178mm x T20mm
Jahr: 2016
Untertitel: Englisch
Auflage: Softcover reprint of the original 2nd ed. 2016

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