Permeability Barrier

This Methods in Molecular Biology(TM) book offers techniques for studying the Permeability Barrier. Includes lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

The significant biological subject, the permeability barrier, is incredibly diverse and vital for a vast assortment of crucial functions in the body. In Permeability Barrier: Methods and Protocols , a variety of experienced researchers contribute techniques to study this complex system in its many forms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Permeability Barrier: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.

Includes cutting-edge methods and protocols covering a variety of permeability barrier Provides step-by-step detail essential for reproducible results Contains key notes and vital implementation advice from the experts

Inhalt

Computational Modeling of the Skin Barrier.- In Vitro Human Skin Segmentation and Drug Concentration-Skin Depth Profiles.- Transcriptional Regulation of Epidermal Barrier Formation.- Epidermal Permeability Barrier (EPB) Measurement in Mammalian Skin.- Assessment of Permeability Barriers to Macromolecules in the Rodent Endometrium at the Onset of Implantation.- Assessment of Intestinal Permeability in (Premature) Neonates by Sugar Absorption Tests.- Analysis of Epithelial Cell Shedding and Gaps in the Intestinal Epithelium.- Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells.- Optimization of the Caco-2 Permeability Assay to Screen Drug Compounds for Intestinal Absorption and Efflux.- Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion.- Monitoring of the Dynamics of Epithelial Dome Formation Using a Novel Culture Chamber for Long-Term Continuous Live-Cell Imaging.- Measuring Permeability in Human Retinal Epithelial Cells (ARPE-19): Implications for the Study of Diabetic Retinopathy.- Analysis of Epithelial Barrier Integrity in Polarized Lung Epithelial Cells.- Permeability of Differentiated Human Urothelium In Vitro.- Phenotyping the Claudin 11 Deficiency in Testis: From Histology to Immunohistochemistry.- An In Vitro System to Study Sertoli Cell Blood-Testis Barrier Dynamics.- Analysis of Endothelial Barrier Function In Vitro.- Role of Endothelial Cell-Cell Junctions in Endothelial Permeability.- In Vitro Analyses of Endothelial Cell Permeability.- Mechano-Transduction and Barrier Regulation in Lung Microvascular Endothelial Cells.- Role of Caveolin-1 in the Regulation of Pulmonary EndothelialPermeability.- Assessment of Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cell Monolayers.- Permeability of Endothelial Barrier: Cell Culture and In Vivo Models.- Size-Selective and In Vitro Assessment of Inner Blood Retina Barrier Permeability.- Assessment of Permeability in Barrier Type of Endothelium in Brain Using Tracers: Evans Blue, Sodium Fluorescein, and Horseradish Peroxidase.- In Vitro and In Vivo Methods for Assessing FcRn-Mediated Reverse Transcytosis Across the Blood-Brain Barrier.- Evaluation of VEGF-Induced Vascular Permeability in Mice.- In Vivo Measurement of Glioma-Induced Vascular Permeability.- In Vivo Optical Imaging of Ischemic Blood Brain Barrier Disruption.