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Permeability Barrier

  • Kartonierter Einband
  • 468 Seiten
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This Methods in Molecular Biology(TM) book offers techniques for studying the Permeability Barrier. Includes lists of necessary ma... Weiterlesen
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Beschreibung

This Methods in Molecular Biology(TM) book offers techniques for studying the Permeability Barrier. Includes lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

The significant biological subject, the permeability barrier, is incredibly diverse and vital for a vast assortment of crucial functions in the body. In Permeability Barrier: Methods and Protocols, a variety of experienced researchers contribute techniques to study this complex system in its many forms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Permeability Barrier: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.

Includes cutting-edge methods and protocols covering a variety of permeability barrier

Provides step-by-step detail essential for reproducible results

Contains key notes and vital implementation advice from the experts



Inhalt

1. Computational Modeling of the Skin Barrier

Arne Naegel, Michael Heisig, and Gabriel Wittum

2. In Vitro Human Skin Segmentation and Drug Concentration-Skin Depth Profiles

Ana Melero, Tsambika Hahn, Ulrich F. Schaefer, and Marc Schneider

3. Transcriptional Regulation of Epidermal Barrier Formation

Ambica Bhandari, Michael L. Salmans, William Gordon, and Bogi Andersen

4. Epidermal Permeability Barrier (EPB) Measurement in Mammalian Skin

Arup Kumar Indra and Mark Leid

5. Assessment of Permeability Barriers to Macromolecules in the Rodent Endometrium at the Onset of Implantation

Brent M. Bany and G. Scot Hamilton

6. Assessment of Intestinal Permeability in (Premature) Neonates by Sugar Absorption Tests

Willemijn E. Corpeleijn, Ruurd M. van Elburg, Ido P. Kema, and Johannes B. van Goudoever

7. Analysis of Epithelial Cell Shedding and Gaps in the Intestinal Epithelium

Carrie A. Duckworth and Alastair J. Watson

8. Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells

Rino P Donato, Adaweyah El-Merhibi, Batjargal Gundsambuu, Kai Yan Mak, Emma R Formosa, Xian Wang, Catherine A Abbott, and Barry C Powell

9. Optimization of the Caco-2 Permeability Assay to Screen Drug Compounds for Intestinal Absorption and Efflux

Barry Press

10. Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion

Isabel Larre, Ruben G. Contreras, and Marcelino Cereijido

11. Monitoring of the Dynamics of Epithelial Dome Formation Using a Novel Culture Chamber for Long-Term Continuous Live-Cell Imaging

Judith Lechner, Daniel Hekl, Helmut Gatt, Markus Voelp, and Thomas Seppi

12. Measuring Permeability in Human Retinal Epithelial Cells (ARPE-19): Implications for the Study of Diabetic Retinopathy

Marta Garcia-Ramírez, Marta Villarroel, Lídia Corraliza, Cristina Hernández, and Rafael Simó

13. Analysis of Epithelial Barrier Integrity in Polarized Lung Epithelial Cells

Monika Strengert and Ulla G. Knaus

14. Permeability of Differentiated Human Urothelium In Vitro

Peter Rubenwolf and Jennifer Southgate

15. Phenotyping the Claudin 11 Deficiency in Testis: From Histology to Immunohistochemistry

Séverine Mazaud-Guittot, Alexander Gow, and Brigitte Le Magueresse-Battistoni

16. An In Vitro System to Study Sertoli Cell Blood-Testis Barrier Dynamics

Dolores D. Mruk and C. Yan Cheng

17. Analysis of Endothelial Barrier Function In Vitro

Yuping Wang and J. Steven Alexander

18. Role of Endothelial Cell-Cell Junctions in Endothelial Permeability

Armelle Le Guelte and Julie Gavard

19. In Vitro Analyses of Endothelial Cell Permeability

Elizabeth Monaghan-Benson and Erika S. Wittchen

20. Mechano-Transduction and Barrier Regulation in Lung Microvascular Endothelial Cells

Kristina Giantsos, Mark Cluff, and Randal Dull

21. Role of Caveolin-1 in the Regulation of Pulmonary Endothelial Permeability

Yu Sun, Richard D. Minshall, and Guochang Hu

22. Assessment of Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cell Monolayers

Andreas Ludwig, Anselm Sommer, and Stefan Uhlig

23. Permeability of Endothelial Barrier: Cell Culture and In Vivo Models

Alexander N. Garcia, Stephen M. Vogel, Yulia A. Komarova, and Asrar B. Malik

24. Size-Selective and In Vitro Assessment of Inner Blood Retina Barrier Permeability

Matthew Campbell and Peter Humphries

25. Assessment of Permeability in Barrier Type of Endothelium in Brain Using Tracers: Evans Blue, Sodium Fluorescein, and Horseradish Peroxidase

Mehmet Kaya and Bulent Ahishali

26. In Vitro and In Vivo Methods for Assessing FcRn-Mediated Reverse Transcytosis Across the Blood-Brain Barrier

Nadia Caram-Salas, Eve Boileau, Graham Farrington, Ellen Garber, Eric Brunette, Abedelnasser Abulrob, and Danica Stanimirovic

27. Evaluation of VEGF-Induced Vascular Permeability in Mice

Sara M. Weis

28. In Vivo Measurement of Glioma-Induced Vascular Permeability

Jisook Lee, Andrew Baird, and Brian P. Eliceiri

29. In Vivo Optical Imaging of Ischemic Blood Brain Barrier Disruption

Abedelnasser Abulrob, Eric Brunette, Jacqueline Slinn, Ewa Baumann, and Danica Stanimirovic

Produktinformationen

Titel: Permeability Barrier
Untertitel: Methods and Protocols
Editor:
EAN: 9781493958627
ISBN: 1493958623
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 468
Gewicht: 873g
Größe: H254mm x B178mm x T25mm
Jahr: 2017
Untertitel: Englisch
Auflage: Softcover reprint of the original 1st ed. 2011

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