PCR

This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field. Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology, and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt
In silico PCR Tools for a Fast Primer, Probe and Advanced Searching.- Introduction on using the FastPCR Software and the Related Java Web Tools for PCR and Oligonucleotide Assembly and Analysis.- Long Fragment Polymerase Chain Reaction.- Strategies to Improve Efficiency and Specificity of Degenerate Primers in PCR.- Inverse PCR for Point Mutation Introduction.- Synthesis of Fusion Genes for Cloning by Megaprimer-based PCR.- A Novel Platform for High-throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli.- Colony PCR.- CRENAME, a Molecular Microbiology Method Enabling Multiparametric Assessment of Potable/Drinking Water.- Multiplex Detection Of Food-borne Pathogen.- Fast Real-time PCR for the Detection of Crustacean Allergens in Foods.- Fast Real-time PCR Method for the Detection of Soy in Foods.- RAPD/SCAR Approaches for Identification of Adulterant's Breed's Milk in Dairy Products.- Genetic Diversity Analysis of Medicinally Important Horticultural Crop Aegle marmelos by ISSR Markers.- PCR in the Analysis of Clinical Samples: Prenatal and Postnatal Diagnosis of Inborn Errors of Metabolism.- Harnessing the Power of PCR-dependent Molecular Fingerprinting Methods for Understanding Structure and Function in Microbial Communities.- PCR in Metagenomics.- Arbitrarily Primed PCR for Comparison of Metagenomes and Extracting useful loci from them. <p