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P-Type ATPases

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This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization ... Weiterlesen
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This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases. P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all researchers in the P-type ATPase field, from the protein biochemist to the mouse geneticist, covering the identification and classification, isolation, purification, in vitro characterization, knock-out studies, as well as crystallization and structural analysis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, P-Type ATPases: Methods and Protocols aims to ensure successful results in the further study of this vital field of research.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

1. An Introduction to P-type ATPase research Poul NissenPart I: Protein Production, Isolation, Purification, and Stabilization2. Purification of Na,K-ATPase from Pig Kidney Natalya U. Fedosova3. Preparation of Ca2+-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum Jesper V. Møller and Claus Olesen4. Isolation of H+/K+-ATPase-enriched Membrane Fraction from Pig Stomachs Kazuhiro Abe and Claus Olesen5. Overproduction of PiB-type ATPasesXiangyu Liu, Oleg Sitsel, Kaituo Wang, and Pontus Gourdon6. Coordinated Overexpression in Yeast of a P4-ATPase and its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex Hassina Azouaoui, Cédric Montigny, Aurore Jacquot, Raphaëlle Barry, Philippe Champeil, and Guillaume Lenoir7. The Plasma Membrane Ca2+-ATPase: Purification by Calmodulin Affinity Chromatography, and Reconstitution of the Purified ProteinVerena Niggli and Ernesto Carafoli 8. Expression of Na,K-ATPase and H,K-ATPase Isoforms With The Baculovirus Expression System Jan B. Koenderink and Herman G.P. Swarts9. Time-dependent Protein Thermostability Assay Ilse Vandecaetsbeek and Peter Vangheluwe Part II : Activity Assays10. Colorimetric Assays of Na,K-ATPase Kathleen J. Sweadner11. ATPase Activity Measurements by an Enzyme-Coupled Spectrophotometric Assay Pankaj Sehgal, Claus Olesen, and Jesper V. Møller12. Antimony-Phosphomolybdate ATPase Assay Gianluca Bartolommei and Francesco Tadini-Buoninsegni13. ATPase Activity Measurements using radiolabeled ATPHerman G.P. Swarts and Jan B. Koenderink14. Assaying P-type ATPases Reconstituted in Liposomes Hans-Jürgen Apell and Bojana Damnjanovic15. Coupling ratio for Ca2+ Transport by Calcium Oxalate PrecipitationPankaj Sehgal, Claus Olesen, and Jesper V. Møller16. Calcium Uptake in Crude Tissue Preparation Philip A. Bidwell and Evangelia G. Kranias17. Measuring H+ Pumping and Membrane Potential Formation in Sealed Membrane Vesicle SystemsAlex Green Wielandt, Michael G. Palmgren, Anja Thoe Fuglsang, Thomas Günther Pomorski, and Bo Højen Justesen18. Assay of Flippase Activity in Proteoliposomes using Fluorescent Lipid Derivatives Magdalena Marek and Thomas Günther Pomorski Part III : In vitro Functional Studies19. The use of Metal Fluoride Compounds as Phosphate Analogs for Understanding the Structural Mechanism in P-type ATPases Stefania J. Danko and Hiroshi Suzuki20. Phosphorylation/Dephosphorylation AssaysHiroshi Suzuki 21. Tryptophan Fluorescence Changes Related to Ca2+-ATPase FunctionPankaj Sehgal, Claus Olesen, and Jesper V. Møller Part IV: Ligand Binding Studies22. Determination of the ATP Affinity of the Sarcoplasmic Reticulum Ca2+-ATPase by Competitive Inhibition of [g-32P]TNP-8N3-ATP Photolabeling Johannes D. Clausen, David B. McIntosh, David G. Woolley, and Jens Peter Andersen23. Ca2+ Binding and Transport Studied with Ca2+/EGTA Buffers and 45Ca2+ Pankaj Sehgal, Claus Olesen, and Jesper V. Møller24. Assay of Copper Transfer and Binding to P1B-ATPases Teresita Padilla-Benavides and José M. Argüello Part V: Electrophysiology25. Voltage Clamp Fluorometry of P-Type ATPases Robert E. Dempski 26. Electrophysiological Measurements on Solid Supported Membranes Francesco Tadini-Buoninsegni and Gianluca Bartolommei 27. Electrophysiological Characterization of Na,K-ATPases Expressed in Xenopus laevis Oocytes Using Two-Electrode Voltage Clamping Florian Hilbers and Hanne Poulsen Part VI: Functional Studies by Cell Culture and Transgenic Animals28. Functional Studies of Na+,K+-ATPase using Transfected Cell Cultures Elena Arystarkhova and Kathleen J. Sweadner29. HPLC Neurotransmitter Analysis Thomas Hellesøe Holm, Toke Jost Isaksen, and Karin Lykke-Hartmann 30. Behavior Test Relevant to a2/a3Na+/K+-ATPase Gene Modified Mouse Models Toke Jost Isaksen, Thomas Hellesøe Holm, and Karin Lykke-Hartmann31. Zebrafish Whole-mount in situ Hybridization Followed by Sectioning Canan Doganli, Jens Randel Nyengaard, and Karin Lykke-Hartmann32. Whole-mount Immunohistochemistry for Anti-F59 in Zebrafish Embryos (1-5 days Post Fertilization (dpf)) Canan Doganli Lucas Bukata and Karin Lykke-Hartmann33. Cell-based Lipid Flippase Assay Employing Fluorescent Lipid Derivatives Maria S. Jensen, Sara Costa, Thomas Günther-Pomorski, and Rosa L. López-Marqués34. Transient Expression of P-type ATPases in Tobacco Epidermal Cells Lisbeth R. Poulsen, Michael G. Palmgren, and Rosa L. López-Marqués Part VII: Lipid Techniques35. Lipid Exchange by UltracentrifugationNikolaj D. Drachmann and Claus Olesen36. Reconstitution of Na+, K+-ATPase in NanodiscsJonas Lindholt Gregersen, Natalya Fedosova, Poul Nissen, and Thomas BoesenPart VIII: Crystallization37. Crystallization of P-type ATPases by the High Lipid-detergent (HiLiDe) Method Oleg Sitsel, Kaituo Wang, Xiangyu Liu, and Pontus Gourdon38. Two-dimensional Crystallization of the Ca2+-ATPase for Electron Crystallography John Paul Glaves, Joseph O. Primeau, and Howard S. Young39. Two-dimensional Crystallization of Gastric H+,K+-ATPase for Structural Analysis By Electron Crystallography Kazuhiro AbePart IX: Computational Approaches in analyzing P-type ATPases40. MD Simulations of P-type ATPases in a Lipid Bilayer System Henriette Elisabeth Autzen and Maria Musgaard41. Computational Classification of P-Type ATPases Dan Søndergaard, Michael Knudsen, and Christian Nørgaard Storm Pedersen42. Molecular Modeling of Fluorescent SERCA Biosensors Bengt Svensson, Joseph M. Autry, and David D. Thomas43. How to Compare, Analyze and Morph Between Crystal Structures of Different Conformations: The P-type ATPase ExampleJesper L. Karlsen and Maike Bublitz


Titel: P-Type ATPases
Untertitel: Methods and Protocols
EAN: 9781493979950
ISBN: 1493979957
Format: Kartonierter Einband
Herausgeber: Springer New York
Anzahl Seiten: 560
Gewicht: 1040g
Größe: H254mm x B178mm x T29mm
Jahr: 2019
Untertitel: Englisch
Auflage: Softcover reprint of the original 1st ed. 2016

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