Nuclear Transfer Protocols

Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis is a comprehensive review of nuclear transfer technology in vertebrates, aimed at reprogramming differentiated nuclei and effecting targeted gene transfer. The emphasis here is on providing readily reproducible techniques for the gene- tion of cloned embryos and animals in a number of key research and commercially important vertebrates. Additional chapters provide alternative cutting-edge methods for nuclear transfer, such as zona-free nuclear transfer and serial nuclear transfer. Of immense practical benefit are descriptions of procedures associated with cloning, such as in vitro maturation of oocytes, activation and culture of cloned embryos, maintenance of pregnancy, and neonatal care of clones. Nuclear Transfer Protocols: Cell Reprogramming and Transgenesis also provides an understanding of the factors involved in nuclear reprogramming, which is imperative for the success of cloning. A section dealing with such cloning-related issues as aging and normality of clones is also included making this an essential comprehensive handbook for research and commercial labo- tories involved in, or intending to work on, nuclear transfer. The volume will prove beneficial to molecular biologists, stem cell biologists, clinicians, biotechnologists, students, veterinarians, and animal care technicians involved with reprogramming, nuclear transfer, and transgenesis.

Includes supplementary material: sn.pub/extras

Inhalt
Nuclear Transfer.- Origin and Progress of Nuclear Transfer in Nonmammalian Animals.- In Vitro Maturation and Embryo Production in Cattle.- Activation of Oocytes After Nuclear Transfer.- Culture Medium Preferences of Pre-Implantation Cloned Mouse Embryos.- Maintenance of Pregnancy in Pigs With Limited Viable Embryos.- Health and Neonatal Care of Bovine Clones.- Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice.- Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors.- A Double Nuclear Transfer Technique for Cloning Pigs.- Nuclear Transfer in Nonhuman Primates.- Cloning of Exotic/Endangered Species: Desert Bighorn Sheep.- Handmade Somatic Cell Cloning in Cattle.- Genetic Modification of Sheep by Nuclear Transfer With Gene-Targeted Somatic Cells.- Transgenesis Using Nuclear Transfer in Goats.- Reprogramming Nuclei.- Nuclear Reprogramming.- Expression of Imprinted Genes in Cloned Mice.- Nuclear Remodeling Assay in Xenopus Egg Extract.- In Vitro Reprogramming of Nuclei and Cells.- Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells.- Gene Expression and Methylation Patterns in Cloned Embryos.- Concluding Remarks.- Are There Any Normal Clones?.- Future and Applications of Cloning.