Non-Viral Gene Delivery Vectors

This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of non-viral gene delivery vectors. The chapters in this book are organized into three major parts: Part I is a section on conventional bolus gene delivery vectors that introduces typical transfection approaches relying on the addition of transfectants to the cell culture medium where the cells are grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or stimuli-responsive polymers that change according to the environment they are in and delivered by dripping into cells; Part III discusses examples of substrate-mediated gene deliveryalso termed reverse transfectionand the immobilization of a gene delivery vector onto a surface as opposed to more typical bolus delivery from the medium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and Protocols is written for experimentalists, and is an essential part of many laboratory bookshelves. This book will help novice and professionals alike succeed in their research in this field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt
Physical Chemical and Biomolecular Methods for the Optimization of Cationic Lipid-Based Lipoplexes In Vitro for the Gene Therapy Applications.- Cationic Lipid-Based Nucleic Acid Vectors.- Lipoplexes from Non-Viral Cationic Vectors: DOTAP-DOPE Liposomes and Gemini Micelles.- Anionic/Zwitterionic Lipid-Based Gene Vectors of pDNA.- Elaboration and Physicochemical Characterization of Niosome-Based Nioplexes for Gene Delivery Purposes.- Quantitative Intracellular Localization of Cationic Lipid-Nucleic Acid Nanoparticles with Fluorescence Microscopy.- Targeted Delivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells.- Lipoplexes Strengthened by Anionic Polymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipids and Anionic Polymers.- Polymer Based Gene Silencing: In Vitro Delivery of SiRNA.- Polyallylamine Derivatives: Novel Non-Toxic Transfection Agents.- Biodegradable Three-Layered Micelles and Injectable Hydrogels.- Cationic Lipid-Coated Polyplexes (Lipopolyplexes) for DNA and Small RNA Delivery.- Preparation of Targeted Anionic Lipid-Coated Polyplexes for MicroRNA Delivery.- Characterization and Investigation of Redox-Sensitive Liposomes for Gene Delivery.- From Artificial Amino Acids to Sequence-Defined Targeted Oligoaminoamides.- Gene Delivery Method Using Photo-Responsive Poly(-Amino Ester) as Vectors.- Thermo-Responsive Polyplex Micelles with PEG Shells and PNIPAM Layer to Protect DNA Cores for Systemic Gene Therapy.- Application of Polyethylenimine-Grafted Silicon Nanowire Arrays for Gene Transfection.