Neural Stem Cells

Many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. This second edition provides the most current, cutting-edge methods of today for the scientists working to answer these questions.

Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. This second edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. Following the Methods in Molecular Biology™ series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls. This is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.

Focus on clinical and translational relevance New and revised chapters that include neural stem cell markers, cytogenetics, and polymerase chain reactions, among others

Klappentext

Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. Neural Stem Cells, 2nd Edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. In addition to the topics covered in the first edition, this new volume provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular Biology™ series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and current, Neural Stem Cells, 2nd Edition is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.

Inhalt
Definitions and Criteria for Stem Cells.- Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard.- Isolation and Culture of NSCs.- Neural Differentiation of Human Embryonic Stem Cells.- Generating Neurons from Stem Cells.- Isolation of Neural Stem and Precursor Cells from Rodent Tissue.- Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization.- Adult Neural Stem Cells.- Olfactory Ensheathing Cells: Isolation and Culture from the Neonatal Olfactory Bulb.- Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium.- Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property.- Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood.- Production of Neurospheres from CNS Tissue.- Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells.- In Vitro Studies: A. Cellular Techniques.- Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures.- Telomere Neurobiology.- Chromosomal Mosaicism in Neural Stem Cells.- In Vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype.- In Vitro Migration Assays of Neural Stem Cells.- Measuring Apoptosis in Neural Stem Cells.- Markers of Adult Neural Stem Cells.- In Vitro Studies: B. Molecular Techniques.- Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR.- Microfluidic Devices for High-Throughput Gene Expression Profiling of Single hESC-Derived Neural Stem Cells.- Electroporation Strategies for Genetic Manipulation and Cell Labeling.- Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches.- Studies for Transplantation.- Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo.- Immunocytochemical Analysis of Neuronal Differentiation.- Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold.- Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS.- Optimizing Stem Cell Grafting into the CNS.- Routes of Stem Cell Administration in the Adult Rodent.