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Natural Killer Cells

  • Kartonierter Einband
  • 384 Seiten
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This volume contains collection of Natural Killer Cell methodologies relevant for both basic and translational research. These met... Weiterlesen
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This volume contains collection of Natural Killer Cell methodologies relevant for both basic and translational research. These methodologies present new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study NK cell migration and cytotoxicity through endpoint analysis or live single cell imaging are also discussed. Chapters also describe methods pertaining to translational application of NK cells, such as ex vivo expansion of NK cells on K562 cell lines genetically modified to express either membrane bound IL-15 or membrane bound IL-21, large scale NK cell culture, current techniques for engineering NK cells to express chimeric antigen receptors or chemokine receptors using retroviral vectors, electroporation of mRNA, and the natural phenomenon of trogocytosis. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Natural Killer Cells: Methods and Protocols is a valuable resource for researchers who not only want to understand mechanisms that govern NK cell behavior and diversity, but also for those who want to understand how to systematically evaluate NK cells for adoptive immunotherapy applications.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras

1. Tracking Effector and Memory NK Cells During MCMV InfectionAimee M. Beaulieu and Joseph C. Sun2. Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer CellsAlexander W. Kay, Dara M. Strauss-Albee, and Catherine A. Blish3. Assessment of NK Cell MetabolismMolly P. Keppel and Megan A. Cooper4. Genotyping Single Nucleotide Polymorphisms and Copy Number Variability of the FCGRs Expressed on NK CellsAmy K. Erbe, Wei Wang, Mikayla Gallenberger, Jacquelyn A. Hank, and Paul M. Sondel5. Measurement of Average Telomere Length in Ex Vivo Expanded Natural Killer Cells by Fluorescence In Situ Hybridization (FISH)Sourindra N. Maiti6. In Vitro Assessment of Human Natural Killer Cell Migration and InvasionKarin Tomin, Ronald H. Goldfarb, and Per Albertsson7. Microfluidic-Based Live-Cell Analysis of NK-Cell Migration In VitroSaravanan Nandagopal, Francis Lin, and Sam K.P. Kung8. Microwell-Based Live Cell Imaging of NK Cell Dynamics to Assess Heterogeneity in Motility and Cytotoxic ResponseBruno Vanherberghen, Thomas Frisk, Elin Forslund, Per E. Olofsson, Karolin Guldevall, and Björn Önfelt9. Assessment of Natural Killer Cell Cytotoxicity Using Image Cytometry MethodKelsey J. McCulley and Srinivas S. Somanchi10. Analysis of Intracellular Ca2+ Mobilization in Human NK Cell Subsets by Flow CytometryJakob Theorell and Yenan T. Bryceson11. Using NK Cell Lipid Raft Fractionation to Understand the Role of Lipid Rafts in NK Cell Receptor SignalingEsther Serrano-Pertierra and Carlos López-Larrea12. High- and Super-Resolution Microscopy Imaging of the NK Cell Immunological SynapseEmily M. Mace and Jordan S. Orange13. The Planar Lipid Bilayer System Serves as a Reductionist Approach for Studying NK Cell Immunological Synapses and their FunctionsGrant Bertolet and Dongfang Liu14. Expansion of NK Cells Using Genetically Engineered K562 Feeder CellsMinh-Trang Thi Phan, Seung-hwan Lee, Sang-Ki Kim, and Duck Cho15. Ex-Vivo Expansion of Human NK Cells Using K562 Engineered to Express Membrane Bound IL21Srinivas S. Somanchi and Dean A. Lee16. Large-Scale Culture and Genetic Modification of Human Natural Killer Cells for Cellular TherapyNatalia Lapteva, Robin Parihar, Lisa A. Rollins, Adrian P. Gee, and Cliona M. Rooney17. Gene Modification of Human Natural Killer Cells Using a Retroviral VectorJoshua N. Kellner, Conrad R. Cruz, Catherine M. Bollard, and Eric S. Yvon18. Modification of Expanded NK Cells with Chimeric Antigen Receptor mRNA for Adoptive Cellular TherapyYaya Chu, Allyson Flower, and Mitchell S. Cairo19. mRNA Transfection to Improve NK Cell Homing to TumorsEmily Levy, Mattias Carlsten, and Richard Childs20. In Vitro Generation of Human NK Cells Expressing Chimeric Antigen Receptor through Differentiation of Gene-Modified Hematopoietic Stem CellsEmily Lowe, Laurel C. Truscott, and Satiro N. De Oliveira21. Engineering Receptor Expression on Natural Killer Cells through TrogocytosisAnitha Somanchi, Dean A. Lee, and Srinivas S. Somanchi22. Electroporation of siRNA to Silence Gene Expression in Primary NK CellsPrasad V. Phatarpekar, Dean A. Lee, and Srinivas S. Somanchi23. Mouse Xenograft Model for Intraperitoneal Administration of NK Cell Immunotherapy for Ovarian CancerDavid L. Hermanson, Laura Bendzick, and Dan S. Kaufman24. Aerosol Delivery of Interleukin-2 in Combination with Adoptive Transfer of Natural Killer Cells for the Treatment of Lung Metastasis: Methodology and EffectSimin Kiany and Nancy Gordon25. Non-Invasive Imaging of Natural Killer Cell-Mediated Apoptosis in a Mouse Tumor Model Thoudam Debraj Singh, Jaetae Lee, and Yong Hyun Jeon26. Non-Invasive In Vivo Fluorescence Imaging of NK Cells in Pre-Clinical Models of Adoptive ImmunotherapySrinivas S. Somanchi27. In Vivo 19F-Magnetic Resonance Imaging of Adoptively Transferred NK CellsSrinivas S. Somanchi, Bridget A. Kennis, Vidya Gopalakrishnan, Dean A. Lee, and James A. Bankson28. Generation of BiKEs and TriKEs to Improve NK Cell-Mediated Targeting of Tumor CellsMartin Felices, Todd R. Lenvik, Zachary B. Davis, Jeffrey S. Miller, and Daniel A. Vallera29. Regulatory Considerations for NK Cells Used in Human Immunotherapy ApplicationsDean A. Lee


Titel: Natural Killer Cells
Untertitel: Methods and Protocols
EAN: 9781493981168
ISBN: 1493981161
Format: Kartonierter Einband
Herausgeber: Springer New York
Anzahl Seiten: 384
Gewicht: 721g
Größe: H254mm x B178mm x T20mm
Jahr: 2018
Untertitel: Englisch
Auflage: Softcover reprint of the original 1st ed. 2016

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