Nanotechnology in Regenerative Medicine

This Methods in Molecular Biology(TM) volume offers methods for applying the principles of nanotechnology to tissue regeneration. Covers delivery systems and biosensors, and techniques for studying interaction between cells, biomolecules and surfaces at nanoscale.

Nanotechnology plays a key leading role in developing tools able to identify, measure, and study cellular events at the nanometric level as well as in contributing to the disclosure of unknown biological interactions and mechanisms, which opens the door for advances including nanodevices for diagnostic and therapy, drug delivery systems, and regenerative medicine. In Nanotechnology in Regenerative Medicine: Methods and Protocols , expert researchers in the field provide an overview of a very wide range of currently used technologies and methods that involve nanotechnology principles applicable to tissue regeneration. Being that the application of nanotechnology to regenerative medicine is a very broad field, this book focuses its interests on particular areas such as its use as a means to produce efficient platforms and structures for tissue engineering, delivery systems and biosensors, as well as the use of some techniques to study materials surfaces and the interactions between cells, biomolecules, and surfaces at the nanoscale. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their related topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Nanotechnology in Regenerative Medicine: Methods and Protocols provides established scientists, junior researchers, and students involved in the bioengineering, biotechnology, and biomedical fields with a sound foundation in a variety of vital nanotechnology approaches in regenerative medicine.

Focuses on interdisciplinary approaches to its cutting-edge subject matter Includes step-by-step protocols that are ready for the lab Features expert tips and implementation advice to ensure successful results Includes supplementary material: sn.pub/extras

Inhalt

Is Nanotechnology the Key to Unravel and Engineer Biological Processes?.- Synthesis of Genetically Engineered Protein Polymers (Recombinamers) as an Example of Advanced Self-Assembled Smart Materials.- Design of Biomolecules for Nanoengineered Biomaterials for Regenerative Medicine.- Stimuli Responsive Polymers for Nanoengineering of Biointerfaces.- Micro-/Nano-Patterning of Proteins Using a Nanoimprint-Based Contact Printing Technique.- Functionalization of Surfaces with Synthetic Oligonucleotides.- Fabrication of PEG Hydrogel Microwell Arrays for High-Throughput Single Stem Cell Culture and Analysis.- Preparation of Polyelectrolyte Nanocomplexes Containing Recombinant Human Hepatocyte Growth Factor (rhHGF) as Potential Oral Carriers for Liver Regeneration.- Electrospinning Technology in Tissue Regeneration.- Protein Adsorption Characterization.- Measuring Wettability of Biosurfaces at the Microscale.- AFM to Study Bio/Non-Bio Interactions.- Evaluation of Cytocompatibility and Cell Response to Boron Nitride Nanotubes.- Nanobiosensors for In Vitro and In Vivo Analysis of Biomolecules.- Novel Strategies to Engineering Biological Tissue In Vitro.- Enabling Biomedical Research with Designer Quantum Dots.- The Role of Nanophotonics in Regenerative Medicine.- Molecular Dynamics Methods for Modeling Complex Interactions in Biomaterials.- Regenerative Nanomedicine: Ethical, Legal, and Social Issue.